MIF、MMP-9在胃癌组织中的表达及临床意义  

Expression and clinical significance of MIF and MMP-9 in gastric cancer

作　　者：包泱 常家聪[1] 王道斌[2] 

机构地区：[1]安徽医科大学第一附属医院普通外科二病区,合肥230022 [2]安徽医科大学第一附属医院中心实验室,合肥230022

出　　处：《安徽医科大学学报》2014年第3期363-366,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学研究项目(编号:KJ2010A181)

摘　　要：目的探讨人胃癌组织及其癌旁正常组织中巨噬细胞移动抑制因子(MIF)和基质金属蛋白酶9(MMP-9)的表达及临床意义。方法选取手术切除并经病理证实的胃癌标本80例作为实验组,另取80例相应癌旁组织作为对照组。应用免疫组化SP法检测癌组织及其癌旁正常组织标本中MIF、MMP-9的表达。结果①胃癌组织中MIF、MMP-9有不同程度的表达,MIF、MMP-9二者的阳性率分别为68.8%和67.5%,均高于癌旁正常组织中的32.5%和37.5%,差异有统计学意义(P<0.05);②MIF的表达与肿瘤浸润深度、分化程度、淋巴结转移及TNM分期呈正相关(P<0.05),与其他临床病理参数无关;MMP-9与肿瘤浸润深度、淋巴结转移及TNM分期呈正相关(P<0.05),与其他临床病理参数无关;③MIF与MMP-9的表达存在关联(χ2=9.154,P<0.01,r=0.338)。结论胃癌组织中MIF与MMP-9均呈阳性表达,且二者的表达呈正相关;二者在胃癌组织中不同程度的表达与胃癌的发生、发展密切相关,并可能指导胃癌的诊断、治疗及预后。Objective To investigate the expression and clinical significance of migration inhibitory factor （ MIF） and matrix metalloproteinase-9（MMP-9） in human gastric cancer tissue and corresponding adjacent normal tissue. Methods Selected surgery and pathologically confirmed 80 cases of gastric cancer as the experimental group, took＆amp;nbsp;another 80 cases of corresponding adjacent tissues as a control group. The expressions of MIF and MMP-9 were de-tected in 80 cases of gastric cancer patients cancer tissue and corresponding adjacent normal tissue by immunohisto-chemical SP method. Results ①The expression of MIF and MMP-9 of gastric cancer in varying degrees：The pos-itive rates of MIF and MMP-9 were 68. 8% and 67. 5%,which were higher than that in adjacent normal tissues, 32. 5% and 37.5% （P 〈0.05）;② The expression of MIF was significantly associated with the clinical stage, depth of invasion,degree of differentiation,lymph node metastasis and TNM staging （P〈0.05）, nothing to do with other clinicopathological parameters;the expression of MMP-9 was significantly associated with the depth of inva-sion,degree of differentiation, lymph node metastasis and TNM staging （P〈0.05）, nothing to do with other clini-copathological parameters;③ There was positive correlation between the expression of MIF and MMP-9 in gastric cancer tissue （χ2 =9. 154, P〈0. 01,r=0.338）. Conclusion The expressions of MIF and MMP-9 show a posi-tive expression in gastric carcinoma, there maybe have a positive correlation between MIF and MMP-9. Their ex-pressions of varying degrees are closely related with the occurrence and development of gastric cancer, guidance gastric diagnosis, treatment and prognosis.

关 键 词：胃癌 MIF MMP-9 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤] R392.112[医药卫生—临床医学]