血小板源性生长因子B及其基因启动子区甲基化在脑胶质瘤中的表达及意义  

Expression of PDGF - B and methylation levels of its gene promoter in human gliomas

作　　者：周益强[1,2] 金贵善[1,2] 米蕊芳[1,2] 董程远[1,2] 张晋[1,2] 刘福生[1,2] 

机构地区：[1]首都医科大学附属北京天坛医院神经外科,100050 [2]北京市神经外科研究所,100050

出　　处：《中华神经外科杂志》2014年第3期288-291,共4页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金项目资助（81071776,81372354,81302186）;北京市自然科学基金项目资助（7102027,7132034）;北京市卫生系统高层次卫生技术人才培养项目资助（2013-2-018）

摘　　要：目的 探讨脑胶质瘤中血小板源性生长因子B（PDGF-B）表达水平及其基因启动子区甲基化程度与肿瘤增殖的关系。方法运用免疫组化检测54例脑胶质瘤中PDGF-B、磷酸化Smad2（P-Smad2）和Ki-67的表达水平；运用焦磷酸测序检测上述54例脑胶质瘤中PDGF-B启动子区甲基化程度。结果（1）PDGF-B表达水平随着病理分级的增高而增多（P〈0．05）；（2）PDGF-B表达分别与Ki-67、P-Smad2表达呈正相关（r=0．839，P〈0．001）（r=0．802，P〈0．001），与PDGF-B甲基化水平呈负相关（r=-0．817，P〈0．001）；（3）在低级别和高级别胶质瘤中，PDGF-B甲基化水平的差异具有统计学意义（P=0．001）。结论PDGF-B甲基化水平越低，其蛋白表达越高，则胶质瘤恶性程度及增殖活性越高。Objective To investigate the role of platelet - derived growth factor B （ PDGF - B） expression levels and the methylation levels of its gene promoter on proliferation of human gliomas. Methods The expressions of PDGF - B, p - Smad2 and Ki - 67 were determined by immunohistoehemistry in 54 human glioma specimens. And the methylation levels of PDGF - B gene promoter were determined using pyrosequencing. Results （ 1 ） The expression levels of PDGF - B increased with the pathologic grades elevated （ P 〈 0. 05 ）. （2） There was a positive and significant correlation between the expression levels of PDGF-Band Ki-67 as well as p-Smad2 （r =0.839, P 〈0.001） （r =0.802, P 〈0.001）, respectively. And an inverse and significant correlation was found between the expression levels of PDGF - B and its gene promoter methylation （ r = - 0. 817, P 〈 0. 001 ）. （3） The methylation levels of PDGF - B gene promoter were significantly higher in low grade gliomas （ WHO Ⅰ Ⅱ ） than those in high grade gliomas （ WHO 11I IV ） （ P = 0. 001 ）. Conclusions The expression levels of PDGF - B and its gene promoter methylation play an important role in predicting the malignancy and proliferation of human gliomas.

关 键 词：神经胶质瘤 基因甲基化 焦磷酸测序 血小板源性生长因子B 

分 类 号：R739.41[医药卫生—肿瘤]