乳腺癌HER-2基因扩增及其蛋白表达与临床病理特征的关系  

HER-2 gene amplification and HER-2/neu protein expression in invasive breast cancer and its relationship to clinicopathological characteristics

作　　者：李世宁[1] 唐红[1] 郭庆喜[1] 阮思蓓[1] 张元[1] 罗霞[1] 孙兴旺[1] 唐明希[1] 

机构地区：[1]泸州医学院附属医院病理科,泸州646000

出　　处：《临床与实验病理学杂志》2014年第3期251-255,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：泸州医学院博士启动基金(2011204);泸州医学院科研启动基金(2012-LG-03);泸州医学院自然科学基金(55)

摘　　要：目的 分析乳腺癌组织中人表皮生长因子受体-2（human epidermal growth factor receptor-2,HER-2）基因扩增与HER-2/neu蛋白表达的一致性与相关性,探讨其与各临床病理特征的关系.方法 采用荧光原位杂交（fluorescent in situ hybridization,FISH）及免疫组织化学（immunohistochemistry,IHC）法分别检测164例浸润性乳腺癌患者的HER-2基因扩增与蛋白表达,分析二者检测结果的一致性、相关性及其临床病理特征的关系.结果 FISH与IHC检测结果符合率为59.75％,结果存在一致性（Kappa=0.099,P=0.008）,且呈正相关（r=0.293,P=0.000）.ER表达与HER-2基因扩增及蛋白表达呈负相关（r=-0.481,P=0.000;r=-0.172,P=0.028）;PR表达及ER/PR状态与HER-2基因扩增呈负相关（r=-0.401,P=0.000;r=-0.382,P=0.000）,与HER-2蛋白表达无关（P＞0.05）.另外,肿瘤大小与HER-2蛋白表达有关（r=0.189,P=0.015）,与HER-2基因扩增状态无关;Ki-67增殖指数、患者年龄、淋巴结转移及肿瘤分级与HER-2基因扩增及蛋白表达均无关.结论 FISH与IHC两种检测结果存在一致性,但IHC（＋～（卅））的患者均应以FISH检测作为评价HER-2基因是否扩增的标准.Purpose To investigate the correlation between human epidermal growth factor receptor-2 （HER-2） gene amplification and HER-2/neu protein expression in invasive breast cancer patients and its relationship to clinicopathological characteristics.Methods Using fluorescence in situ hybridization （FISH） and immunohistochemistry （IHC） method,the HER-2 gene amplification and HER-2/neu protein expression status were detected,respectively,and its concordance and correlation,as well as their relationships to the clinical characteristics were analyzed in a total of 164 samples of invasive breast cancer tissues.Results The overall coincidence rate of FISH and IHC was 59.75 ％ （Kappa =0.099,P =0.008）,with positive correlation （r =0.293,P =0.000） between them.The expression of ER （estrogen receptor） was negatively correlated with HER-2 gene amplification and the expression of HER-2 protein （r =-0.481,P =0.000 and r =-0.172,P =0.028）.The PR expression and ER/PR status was negatively correlated with HER-2 gene amplification （r =-0.401,P =0.000 and r =-0.382,P =0.000）,but without relevant to the HER-2 protein expression （P ＞0.05）.In addition,the tumor size was positive correlation with HER-2 protein expression （r =0.189,P =0.015）,but not with HER-2 gene amplification.Ki-67,age,lymph node metastasis and tumor grade status were not correlated with the HER-2 gene amplification and its protein expression.Conclusion There is a concordance between the HER-2 gene amplification and its protein expression.HER-2 gene amplification status should be further detected by FISH in patients with HER-2 protein expression （＋ ～ （井））.

关 键 词：乳腺肿瘤 人表皮生长因子受体-2 荧光原位杂交 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]