乳腺癌中高危型人乳头瘤病毒感染及其与微血管密度的相关性  

Relationship of microvessel density and high-risk human papilloma virus infection in breast cancer

作　　者：胡仲翔[1] 李德群[1] 岳喜成[1] 胡闻[2] 

机构地区：[1]蚌埠医学院第一附属医院肿瘤外科,蚌埠233004 [2]安徽省立医院病理科,合肥230001

出　　处：《临床与实验病理学杂志》2014年第3期261-263,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨人乳头瘤状病毒(human papilloma viruses,HPV)感染在乳腺癌中的表达及其与肿瘤微血管密度(microvessel density,MVD)的关系。方法应用免疫组化PV 6000法检测90例乳腺浸润性导管癌组织(NOS)、23例癌旁组织、40例乳腺腺病组织以及20例正常乳腺组织中CD34表达;采用原位分子杂交法检测相应样本组织中HPV16/18 DNA分子。结果乳腺浸润性导管癌组织中MVD(44.18±11.96)明显高于乳腺癌旁组织(24.70±10.33)、乳腺腺病组(21.35±8.47)及正常乳腺组(12.63±7.12)(P<0.05);HPV16/18在乳腺浸润性导管癌组织中阳性率为35.6%(32/90),乳腺癌旁组织、乳腺腺病组及正常乳腺组HPV16/18阳性率分别为17.4%(4/23)、12.5%(5/40)和10%(2/20),明显低于乳腺癌组(P<0.05);HPV16/18DNA表达与患者年龄、月经状况、肿瘤大小、分级及淋巴结转移无关(P>0.05);HPV16/18阳性组MVD明显高于HPV16/18阴性组(P<0.05)。结论 HPV16/18 DNA表达在乳腺癌发生、发展中具有一定的作用,HPV16/18 DNA表达与肿瘤内MVD有相关性。Purpose To investigate the expression of human papilloma virus （HPV） in carcinoma of the breast and its relationship with microvessel density （MVD） and clinic pathological parameters.Method CD34 and HPV16/18 were detected by immunohistochemistry PV 6000 and in situ hybridization in 90 cases of breast cancer,23 cases of breast cancer adjacent tissues,40 cases of breast adenosis and 20 cases of normal breast tissues.Results MVD in breast cancer （44.18 ＋ 11.96） was significantly higher than that in paracancerous tissues （24.7 ± 10.33）,breast adenosis （21.35 ± 8.47） and normal breast tissues （12.63 ＋ 7.12） （P ＜ 0.05）,MVD was correlated with lymphatic metastasis.The positive rate of HPV16/18 expression was 35.6％ （32/90） in breast cancer tissues.There were statistical differences in paracancerous tissues （17.4％,4/23）,breast adenosis （12.5％,5/40） and normal breast tissues （10％,2/20） （P ＜ 0.05）.The expressions of HPV16/18 in breast cancer tissues were positively associated with MVD （P ＜ 0.05）,but not with age,menopause,tumor size,staging and other clinic pathological characteristics （P ＞ 0.05）.Conclusion HPV16/18 plays an important role in the carcinogenesis of breast cancer,and have some relationship with MVD.

关 键 词：乳腺肿瘤 HPV病毒 CD34 微血管密度 原位分子杂交 

分 类 号：R737.9[医药卫生—肿瘤]