Cloning the Dmrt1 and DmrtA2 genes of ayu(Plecoglossus altivelis) and mapping their expression in adult,larval,and embryonic stages  

作　　者：Jin-Hua WANG Liang MIAO Ming-Yun LI Xiao-Fei GUO Na PAN Ying-Ying CHEN Liang ZHAO 

机构地区：[1]Ningbo University

出　　处：《Zoological Research》2014年第2期99-107,共9页动物学研究（英文）

基　　金：Special Preliminary Study of 973(2008CB117015);Changjiang Scholars and Innovative Research Team Projects(IRT0734);Priority Themes of Major Science and Technology in Zhejiang Province(2009C12077);the K.C.Wong Magna Fund of Ningbo University

摘　　要：The Dmrt family of genes are involved in sex differentiation in different species of invertebrates, and some vertebrates including human. In this study, we cloned the full-length eDNA of ayu （Plecoglossus altivelis） Draft1 and DmrtA2. Sequence and phylogenetic tree analyses showed ayu Dmrtl showed highest similarity to that of Oncorhynchus mykiss while ayu DmrtA2 is most similar to that of Oryzias latipes. Fluorescence-based quantitative reverse transcription PCR （qRT-PCR） revealed the Draft1 was predominantly expressed in the testis. At the larval stages, Dmrtl mRNA expression level was highest during 52-64 days post hatching （dph） and at the gastrula stage during embryonic development. DmrtA2, meanwhile, was specifically expressed in the ovary and was highly expressed in the female brain tissue, but not male brain tissue. During the larval stages, DmrtA2 expression remained high before day 34, and then fluctuated while generally decreasing. During embryonic development, DmrtA2 expression increased gradually and peaked at the hatching stage. Our data suggest that ayu Dmrtl might participate in the differentiation and maintenance of testis while DmrtA2 may play a role in ovary-differentiation and mature-ovary maintenance. DmrtA2 might also participate in brain development.The Dmrt family of genes are involved in sex differentiation in different species of invertebrates, and some vertebrates including human. In this study, we cloned the full-length eDNA of ayu （Plecoglossus altivelis） Draft1 and DmrtA2. Sequence and phylogenetic tree analyses showed ayu Dmrtl showed highest similarity to that of Oncorhynchus mykiss while ayu DmrtA2 is most similar to that of Oryzias latipes. Fluorescence-based quantitative reverse transcription PCR （qRT-PCR） revealed the Draft1 was predominantly expressed in the testis. At the larval stages, Dmrtl mRNA expression level was highest during 52-64 days post hatching （dph） and at the gastrula stage during embryonic development. DmrtA2, meanwhile, was specifically expressed in the ovary and was highly expressed in the female brain tissue, but not male brain tissue. During the larval stages, DmrtA2 expression remained high before day 34, and then fluctuated while generally decreasing. During embryonic development, DmrtA2 expression increased gradually and peaked at the hatching stage. Our data suggest that ayu Dmrtl might participate in the differentiation and maintenance of testis while DmrtA2 may play a role in ovary-differentiation and mature-ovary maintenance. DmrtA2 might also participate in brain development.

关 键 词：AYU Dmrt Sequence analysis EXPRESSION 

分 类 号：S917.4[农业科学—水产科学]