胰腺癌早期诊断相关miRNAs的筛选与验证  

Application of miRNAs in the occurrence and early diagnosis of pancreatic cancer

作　　者：夏蜀珺[1] 郭俊超[1] 李建[1] 周立[1] 赵玉沛[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院基本外科, 100730

出　　处：《中华外科杂志》2014年第3期198-201,共4页Chinese Journal of Surgery

基　　金：卫生行业科研专项资助项目（201202007）

摘　　要：目的 寻找具有潜在早期诊断价值的外周血特异性miRNAs；探索胰腺癌发生过程中相关miRNAs水平的动态变化及其对胰腺癌早期诊断的价值.方法 通过建立SD大鼠胰腺癌演进模型,利用miRNA芯片技术筛选胰腺癌发生相关的差异miRNA表达谱,并利用生物信息学技术对相关miRNAs进行初步验证分析.结果 胰腺、血标本中分别获得有意义的差异表达miRNAs 35条和12条,验证结果发现hsa-let-7c、hsa-miR-122-5p、hsa-miR-142-5p、hsa-miR-199a-3p及hsa-miR-451a在正常胰腺与胰腺癌之间表达差异无统计学意义（P＞0.05）.结论 miRNAs与胰腺癌的发生密切相关,有潜力成为早期胰腺癌血清特异性肿瘤标记物.初步建立的早期胰腺癌差异miRNAs表达谱为进一步研究胰腺癌发生分子机制奠定了基础.Objective To find the potential serum specific miRNAs with diagnostic value in early pancreatic cancer and study the alteration of miRNAs levels in the process of origin and development of pancreatic cancer and discuss the diagnostic value of miRNAs in early pancreatic cancer.Methods DMBA-induced rat model was established.The miRNAs expression profile of early stage was screened out by microarray.And confirmation study was performed.Results The 35 and 12 abnormally expressed miRNAs were acquired in pancreatic tissue and blood respectively.There were no significant differences between normal pancreas and pancreatic cancer in expressions of hsa-let-7c,hsa-miR-122-5p,hsa-miR-142-5p,hsamiR-199a-3p and hsa-miR-451 a （P 〉0.05）.Conclusions miRNAs are the potential biomarkers of early pancreatic cancer.The establishment of the miRNAs expression profile has build the foundation of exploring the molecular mechanism of origin of pancreatic cancer.

关 键 词：胰腺肿瘤 疾病模型 动物 

分 类 号：R735.9[医药卫生—肿瘤]