牙龈卟啉单胞菌感染对单核细胞与人脐静脉内皮细胞黏附的影响  

Porphyromonas gingivalis infection enhanced the adhesion of monocytes to endothelial cells

作　　者：张彩霞[1] 宋洁[1] 黎景景 高鑫[1] 孙卫斌[1] 李宽钰[1] 

机构地区：[1]南京大学医学院细胞生物学和遗传学教研室·江苏省医学分子技术重点实验室,210093

出　　处：《中华口腔医学杂志》2014年第3期151-154,共4页Chinese Journal of Stomatology

基　　金：南京大学医药生物技术国家重点实验室开放研究基金（KF-GN-201108）

摘　　要：目的 观察牙龈卟啉单胞菌（Porphyromonas gigivalis,Pg）感染单核细胞后是否增加单核细胞对人脐静脉内皮细胞（human umbilical vein endothelial cell,HUVEC）的黏附并探讨其分子机制,为从牙周病的角度预防动脉粥样硬化的发生提供新的研究思路.方法 应用厌氧罐培养Pg,以感染复数为1∶100感染人单核细胞株THP-1.感染0（对照组）、8和24h后与HUVEC共培养1h,去除未黏附的THP-1后观察THP-1对HUVEC的黏附,再培养23 h后用酶联免疫吸附测定法检测相应细胞因子的表达及黏附分子的变化.结果 Pg感染24h后,THP-1对HUVEC的黏附较对照组提高了13.8％ ～35.2％ （P =0.006）;细胞间黏附分子1的表达量[（164.9±9.1） ng/L]显著高于对照组[（132.5±7.7） ng/L] （P =0.005）;单核细胞趋化蛋白1的分泌量[（221.0±4.9）ng/L]显著高于对照组[（183.2±3.1）ng/L]（P=0.012）;细胞分泌白细胞介素8的量[（787.2±10.3） ng/L]亦显著高于对照组[（587.2±5.1）ng/L] （P=0.002）.结论 Pg感染促进单核细胞与HUVEC的黏附;Pg感染可能是动脉粥样硬化发生的危险因素.Objective To observe if Porphyromonas gingivalis （Pg） infection could enhance the adhesion of human monocytic cell line （THP-1） to human umbihcal vein endothelial cells （HUVEC）.Methods PgATCC33277 was cultured in anaerobic jar,and THP-1 was infected with various concentrations of PgATCC33277 at multiplicity of infection（MOI） of 1：100 for 8 and 24 hours,respectively.Mter removal of the free Pg,THP-1 cells were cocultured with HUVEC for 1 hour to observe the adhesion of THP-1 to HUVEC.HUVEC with adhesive THP-1 cells were co-cultured for additional 23 hours.The medium and cells were separately collected.The expression of related chemotactic cytokine [monocyte chemotactic protein 1 （MCP-1） and interleukin 8 （IL-8）] and intercellular adhesion molecule-1 （ICAM-1） were detected with enzyme-linked immunosorbent assay.Results The adhesion of THP-1 to HUVEC was enhanced （13.8％-35.2％,P=0.006） and the expression of ICAM-1 of HUVEC was increased from （132.5 ±7.7） to （164.9 ±9.1） ng/L （P=0.005） after infection for 24 hours by Pg.Both of the secreted MCP-I and IL-8 elevated after infection of Pg for 24 hours from （183.2 ± 3.1） to （221.0 ± 4.9） ng/L （P =0.012） and from （587.2 ±5.1） to （787.2 ± 10.3） ng/L （P =0.002）,respectively.Conclusions Pg could enhance the adhesion of monocytes to endothelial cells and stimulate the inflammation,suggesting that Pg infection may be one of the risk factors in promoting the development of atherosclerosis.

关 键 词：紫单胞菌 龈 单核细胞 内皮细胞 细胞粘附分子 

分 类 号：R781.4[医药卫生—口腔医学]