激光诱导的恒河猴脉络膜新生血管模型的研究  

The study of laser-induced choroidal neovascularization in rhesus monkeys

作　　者：赖坤贝[1] 金陈进[1] 涂姝[1] 熊云帆[1] 黄蕊[1] 葛坚[1] 

机构地区：[1]中山大学中山眼科中心眼科学国家重点实验室, 广州510060

出　　处：《中华眼科杂志》2014年第3期203-208,共6页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金

摘　　要：目的 探讨激光诱导的恒河猴CNV模型的形态学和功能学变化.方法 实验研究.8只体重4～7 kg的成年恒河猴用于该研究.采用高能量、短曝光时间和小光斑直径的氩激光光凝视网膜诱导CNV.通过彩色眼底拍照、FFA和OCT等方法对恒河猴CNV的自然病程进行连续观测.采用单因素重复测量设计资料的方差分析法对FFA检查的4级光斑荧光素渗漏强度进行统计分析,同时采用配对t检验的方法对激光建模前及激光光凝建模后56 d恒河猴CNV模型视觉电生理各参数的改变进行统计分析.结果 （1）FFA检查结果显示激光光凝后14、21、28、35、42、49、56 d的4级光斑平均荧光素渗漏强度的灰度值分别为89.44±26.28、97.56±26.47、110.22±29.76、100.26±29.24、91.77±28.11、77.76±24.85、63.23±22.34,差异有统计学意义（F =39.715,P ＜0.01）.每个时间点的荧光素渗漏强度与前一个时间点的荧光素渗漏强度相比,差异均有统计学意义（t14～21=4.824,P＜0.01; t21～28=5.225,P＜0.01; t28-35=7.378,P＜0.01;t35 ～42=2.954,P＜0.05; t42～49=5.386,P＜0.01;t49～56=6.138,P＜0.01）.（2）OCT检查结果可见光斑处有代表CNV形成的红色高反射光团,并伴有视网膜水肿及视网膜下积液.组织学HE染色检查结果证实CNV形成并可见较多色素细胞增殖、移行至CNV边界.（3）视网膜电图检查数据显示,与建模前相比,激光建模后56 d暗适应b波潜伏期延长（=4.23,P＜O.01）,暗适应a波振幅（t=6.35,P＜0.01）、暗适应b波振幅（t=3.12,P＜0.01）及明适应b波振幅（t=3.93,P＜0.01）均下降,差异有统计学意义.结论 激光诱导的恒河猴CNV模型同时具备持续荧光素渗漏的形态学特点和潜伏期延长、振幅下降的功能学改变特点,可用于CNV发病机制的研究及CNV治疗药物有效性的筛选.Objective To investigate the morphological and functional changes of the laser-induced choroidal neovascularization（CNV） in rhesus monkeys.Methods Experimental study.Eight adult rhesus monkeys weighted 4 to 7 kg were used in this study.CNVs were induced with small high-energy laser spots at short pulse duration by an argon green laser.Eyes were monitored weekly by color fundus photography,fluorescence fundus angiography （FFA）,and optical coherence tomography （OCT）.Fluorescein leaking intensities of grade 4 CNV lesions were analyzed by the method of ANOVA for repeated measures.Electroretinogram（ERG） was performed before laser photocoagulation and 56 days after laser photocoagulation and the data were analyzed with paired t-test.Results （1） FFA revealed that the mean intensities of grade 4 CNV lesions were 89.44 ± 26.28,97.56 ± 26.47,110.22 ± 29.76,100.26 ± 29.24,91.77 ± 28.11,77.76±24.85 and 63.23 ±22.34 on day 14,day 21,day 28,day 35,day 42,day 49,and day 56 respectively and the differences were statistically significant（F =39.715,P 〈 0.01）.The differences between any time-point and its previous time-point were also statistically significant （t14-21 =4.824,P 〈 0.01 ;t21-28 =5.225,P 〈 0.01 ;t28-35 =7.378,P 〈 0.01 ; t35-42 =2.954,P 〈 0.05 ; t42-49 =5.386,P 〈 0.01 ; t49-56 =6.138,P 〈0.01）.（2）OCT images showed retinal edema,subretinal fluid and hyper-reflective lesions of CNVs inthe laser sites and histopathology showed that fibrovascular tissues together with proliferating retinal pigment epithelium cells were seen in the laser sites.（3） ERG data revealed that implicit time of dark-adapted b wave （t =4.23,P 〈 0.01） increased while the amplitudes of dark-adapted a wave （t =6.35,P 〈 0.01）,darkadapted b wave（t =3.12,P 〈 0.01） and light-adapted b wave （t =3.93,P 〈 0.01） decreased 56 days after laser photocoagulation compared with those before laser photocoagulation.Conelusion The laser-induced CNV in non-human primate model shows continuous

关 键 词：脉络膜新生血管化 疾病模型 动物 恒河猴 

分 类 号：R-332[医药卫生] R773.4