Regulation network and expression profiles of Epstein-Barr virus-encoded microRNAs and their potential target host genes in nasopharyngeal carcinomas  

作　　者：ZENG ZhaoYang HUANG HongBin HUANG LiLi SUN MengXi YAN QiJia SONG YaLi WEI Fang BO Hao GONG ZhaoJian ZENG Yong LI Qiao ZHANG WenLing LI XiaYu XIANG Bo LI XiaoLing LI Yong XIONG Wei LI GuiYuan 

机构地区：[1]Hunan Provincial Tumor Hospital,Xiangya School of Medicine,Central South University [2]Key Laboratory of Carcinogenesis of Ministry of Health,Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute,Central South University [3]Hunan Key Laboratory of Nonresolving Inflammation and Cancer,Disease Genome Research Center,The Third Xiangya Hospital,Central South University [4]Key Laboratory of Information System Engineering,National University of Defense Technology [5]Department of Biochemistry and Molecular Biology and Center for Genetics and Molecular Medicine,School of Medicine,University of Louisville

出　　处：《Science China(Life Sciences)》2014年第3期315-326,共12页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(81172189,81171930,81272298,81272254,91229122,81301757,81372907);the Natural Science Foundation of Hunan Province(14JJ1010);the Fundamental Research Funds for the Central Universities(2011JQ020);the Postdoctoral Science Foundation of Central South University

摘　　要：Epstein-Barr virus(EBV)is associated with nasopharyngeal carcinoma(NPC)tumorigenesis.However,the mechanism(s)connecting EBV infection and NPC remain unclear.Recently,a new class of EBV microRNAs(miRNAs)has been described.To determine how EBV miRNAs control the expression of host genes,and to understand their potential role in NPC tumorigenesis,we profiled the expression of 44 mature EBV miRNAs and potential host genes in NPC and non-tumor nasopharyngeal epithelial tissues.We found that 40 EBV miRNAs from the BART transcript were highly expressed in NPC.Analysis of potential BART miRNA target genes revealed that 3140 genes and several important pathways might be involved in the carcinogenesis of NPC.A total of 105 genes with potential EBV miRNA binding sites were significantly downregulated,suggesting that EBV miRNAs may regulate these genes and contribute to NPC carcinogenesis.An EBV miRNA and host gene regulation network was generated to provide useful clues for validating of EBV miRNA functions in NPC tumorigenesis.Epstein-Barr virus （EBV） is associated with nasopharyngeal carcinoma （NPC） tumorigenesis. However, the mechanism（s） connecting EBV infection and NPC remain unclear. Recently, a new class of EBV microRNAs （miRNAs） has been described. To determine how EBV miRNAs control the expression of host genes, and to understand their potential role in NPC tumor- igenesis, we profiled the expression of 44 mature EBV miRNAs and potential host genes in NPC and non-tumor nasopharyn- geal epithelial tissues. We found that 40 EBV miRNAs from the BART transcript were highly expressed in NPC. Analysis of potential BART miRNA target genes revealed that 3140 genes and several important pathways might be involved in the car- cinogenesis of NPC. A total of 105 genes with potential EBV miRNA binding sites were significantly downregulated, sug- gesting that EBV miRNAs may regulate these genes and contribute to NPC carcinogenesis. An EBV miRNA and host gene regulation network was generated to provide useful clues for validating of EBV miRNA functions in NPC tumorigenesis.

关 键 词：Epstein-Barr virus （EBV） microRNA （miRNA） nasopharyngeal carcinoma （NPC） gene regulate network 

分 类 号：R739.63[医药卫生—肿瘤]