改性硅藻土对脂肪酶固定化研究  

Study on modified diatomite for lipase immobilization

作　　者：刘媛媛[1] 郑永杰[1] 田景芝[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《化学工程师》2014年第3期14-17,共4页Chemical Engineer

基　　金：齐齐哈尔大学青年教师科研启动支持计划资助(2010K-M24);黑龙江省教育厅资助项目(12521612)

摘　　要：以改性硅藻土为载体,采用吸附法对脂肪酶进行固定化。对固定化条件进行优化。得到较佳的固定化条件:固定化时间为4h;温度为40℃;pH值为7.5;固定化酶在40℃下保温2h后酶活损失很小,固定化酶重复性达到最佳状态。The lipase was immobilized by adsorption method with modified diatomite as carrier. The optimized conditions were immobilized time is 4h, temperature is 40℃, pH value is 7.5. The results showed that enzyme activity loss was little when under 40℃ and heat preserved 2h. The immobilized enzyme repeatability achieve the best.

关 键 词：改性硅藻土 固定化 脂肪酶 

分 类 号：Q814.2[生物学—生物工程]