羧酸酯酶1对大鼠颈动脉损伤后血管狭窄及炎性反应的影响  

Effect of carboxylesterase 1 on stenosis and inflammatory response in rats after carotid artery injury

作　　者：甄为[1] 罗俊生[1] 霍晓川[1] 关宁[1] 王之赫 邱建武[1] 

机构地区：[1]辽宁医学院,辽宁锦州121001

出　　处：《解放军医学院学报》2014年第3期261-265,共5页Academic Journal of Chinese PLA Medical School

基　　金：国家自然科学基金项目(81171111);辽宁省教育厅科学研究项目(L2012298);辽宁省科技厅自然基金项目(2013022016)~~

摘　　要：目的探讨羧酸酯酶1(carboxylesterase 1,CES1)对大鼠颈动脉内膜损伤后血管狭窄及炎症反应的影响。方法随机将大鼠分为3组,CES1组使用球囊导管制备颈总动脉损伤模型,在术后0 d、1 d、2 d尾经脉注射载有CES1的慢病毒载体促进CES1高表达;空载病毒组颈动脉损伤后注射空载病毒;空白对照组给予假手术处理。于术后第14天取材,HE染色后计算内膜增生情况,免疫组化和Western Blot检测TLR-4蛋白表达,实时荧光定量PCR测定TRL-4基因表达。结果HE染色表明CES1高表达能够明显抑制血管内膜损伤后的内膜增生,降低炎症反应,减少血管狭窄,免疫组化及实时荧光定量PCR和Western blot结果显示CES1高表达能够降低血管内膜中TLR-4的蛋白及基因表达。结论 CES1高表达能够下调损伤血管内TLR-4的表达从而抑制炎症反应,减轻血管损伤后狭窄。Objective To study the effect of carboxylesterase 1 （CES1） on stenosis and inflammatory reactions in rats after carotid artery injury. Methods Rats were randomly divided into CES1 group, non-virus loading group and blank control group. Rats in CES1 group were injected with slow virus vectors containing CES1 via the tail vein on days 0, 1, and 2 after the common carotid artery injury model was established. Rats in non-virus loading group were injected with slow virus vectors containing no CES1 after carotid artery injury. Rats in control group underwent sham operation. Carotid artery tissue samples were taken on day 14 after carotid artery injury. Hyperplasia of carotid artery intima was assayed with H＆amp;E staining. Expression of TLR-4 was detected by Western blot and RT-PCR, respectively, with immunohistochemical staining. Results Over-expressed CES1 could significantly inhibit hyperplasia of carotid artery intima, improve inflammatory reactions and reduce stenosis. RT-PCR and Western blot showed that over-expressed CES1 could down-regulate the expression of TLR-4 in carotid artery intima. Conclusion Over-expressed CES1 can inhibit the inflammatory reactions and improve stenosis after carotid artery injury by down-regulating the expression of TLR-4.

关 键 词：羧酸酯酶 炎症 TLR-4 血管损伤 TLR-4 

分 类 号：R363[医药卫生—病理学]