抑癌方对结肠癌转移潜能及血管生成拟态的影响  

Effects of Yiai Decoction on Metastatic Potential and Vasculogenic Mimicry of Colon Cancer Cells

作　　者：刘见荣[1] 徐燕丰[1] 可飞 李文[1] 管宇[1] 罗梅宏[1] 李明花[1] 侯风刚[1] 

机构地区：[1]上海中医药大学附属市中医医院,上海200071 [2]东南大学医学院病理与病理生理学系,江苏南京210009

出　　处：《辽宁中医杂志》2014年第3期574-576,I0004,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81173221);上海教委科研项目(2012JW58)

摘　　要：目的:观察结肠癌细胞的转移潜能(迁移、侵袭力)是否和血管生成拟态(VM)存在相关性;探讨抑癌方对结肠癌转移潜能的影响,及其机制是否与VM有关。方法:以实验中可以形成VM的人结肠癌细胞为VM(+)组,不能形成VM的人结肠癌细胞为VM(-)组,划痕、Transwell侵袭实验观察其迁移、侵袭能力;对VM(+)组细胞进行后续实验,分组如下:抑癌方处理过的中药组和未经处理的空白对照组,划痕、侵袭实验观察其迁移、侵袭能力,体外三维培养法观察其血管生成拟态的形成情况。结果:三维培养环境下,人结肠癌细胞株HCT116可以形成VM为VM(+)组,HT29不能形成VM为VM(-)组,VM(+)组的迁移、侵袭力明显强于VM(-)组(P<0.05),且VM密度和细胞的迁移呈正相关(r=0.994,P<0.05),VM密度和侵袭力呈显著正相关(r=0.998,P<0.05);经抑癌方处理的中药组其迁移、侵袭力明显弱于未处理的对照组(P<0.05),中药组的VM密度和对照组相比差异有统计学意义(P<0.05)。结论:结肠癌的迁移、侵袭能力和VM存在相关性,抑癌方可以抑制结肠癌细胞的迁移和侵袭能力,其机制可能和抑制VM的形成有关。Objective：To observe the relationship between VM and the migration and invasion in colon cancer cells, and inves- tigate whether Yiai Decoction could inhibit the metastatic potential of human colon cancer cell lines by inhibiting the formation of VM. Methods ：The colon cancer cell lines which can form VM were considered as VM positive group, while those having no this capability were Called VM negative group. Then the migration and invasion of colon cancer cells were detected by wound healing assay and invasion assay. In addition, VM positive group ceils were treated with Yiai Decoction in subsequent experiments. So there were two groups：blank control group and medicines group. We observed the effects of Yiai Decoction on the migration and invasive capacity of colon cancer ceils and the forming of VM. Results ： HCT116 cells could form visible VM structure when cul- tured in three -dimensional condition and were considered as VM positive group ,while HT29 cells which were attributable to VM negative group could not. The migration and invasive capacity of VM positive group were stronger than VM negative group. Fur- thermore, we found that the VM density was both positively correlated with migration（ r = 0.994, P 〈 0.05 ） and invasive capacity of colon cancer cells （ r = 0. 998, P 〈 0.05 ）. Compared with control group, medicine group had weaker migration and invasive capacity, together with less VM density. Conclusion ： In colon cancer cells, VM may be contributed to cell migration and invasion. Yiai Decoction attenuated the migration and invasion ability of colon cancer cells probably by inhibiting VM formation.

关 键 词：抑癌方 结肠癌 血管生成拟态 迁移 侵袭 

分 类 号：R735.35[医药卫生—肿瘤]