不同活血方剂对高转移人肝癌裸鼠原位移植瘤生长和转移及肿瘤血管生成的影响  

Effect of different blood-promoting formulas in the tumor-genesis, metastasis and angiogenesis of nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential

作　　者：闵亮[1] 祁宏[1] 

机构地区：[1]上海交通大学医学院附属仁济医院中医科,上海200127

出　　处：《中国中西医结合消化杂志》2014年第3期113-117,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：上海市卫生局中医药专项基金资助项目(No:2010J009A)

摘　　要：[目的]观察不同活血方剂对高转移人肝癌裸鼠原位移植瘤生长、转移及肿瘤血管生成的影响。[方法]建立高转移人肝癌裸鼠原位移植瘤模型HCCLM3,随机分为5组:模型组、大黄蛰虫丸组、补阳还五汤组、血府逐瘀汤组、桂枝茯苓丸组,每组8只。于造模第2天起开始给药。模型组以0.9%氯化钠溶液灌胃,其余各组以相应药液灌胃,1次/d,连续35d。观察各组小鼠生存状况及移植瘤生长情况,称量瘤重,计算抑瘤率,ELISA法检测各组小鼠血清血管内皮生长因子(VEGF)表达水平,免疫组化法检测肿瘤微血管密度,并检测肿瘤肺转移情况。[结果]与模型组比较,大黄蛰虫丸组、补阳还五汤组瘤重显著降低(P<0.05),而血府逐瘀汤组、桂枝茯苓丸组瘤重无明显变化(P>0.05);大黄蛰虫丸组、补阳还五汤组、血府逐瘀汤组、桂枝茯苓丸组抑瘤率分别为41.76%、51.9%、2.14%、3.46%;与模型组比较,大黄蛰虫丸组血清VEGF水平及肿瘤微血管密度显著增高(P<0.05),而补阳还五汤组、血府逐瘀汤组、桂枝茯苓丸组无明显变化(P>0.05);与模型组比较,大黄蛰虫丸组肺转移灶数显著增加(P<0.05),补阳还五汤组显著减少(P<0.05),而血府逐瘀汤组、桂枝茯苓丸组无明显变化(P>0.05)。[结论]大黄蛰虫丸可抑制原位移植瘤的生长,但促进了肿瘤血管生成和肿瘤肺转移;补阳还五汤可抑制移植瘤的生长和肺转移,对肿瘤血管生成未见明显的影响;血府逐瘀汤、桂枝茯苓丸对移植瘤的生长、转移及肿瘤血管生成没有明显的影响。[Objective]To investigate the effect of different blood-promoting formulas in the tumor-genesis,metastasis and angiogenesis of nude mice bearing human hepatocellular carcinoma （HCC） xenografts.[Methods] Metastatic human HCC nude mice models were established by orthotopic implantation with a high metastatic potential human HCC cell line HCCLM3.Forty nude mice bearing HCCLM3 were randomly divided into 5 groups：the control group,the Da-Huang-Zhe-Chong Pill group,the Bu-Yang-Huan-Wu Decoction group,the Xue-Fu-Zhu-Yu Decoction group,the Gui-Zhi-Fu-Ling Pill group,8 mice in each group.Since the day after modeling,the decoctions were given to the mice in the treatment groups by ga-vage respectively once a day for 35 days,while the doses by gavage in the control group were normal saline for the same duration.The changes among the groups in the living status of mice,the tumor growth of HCC and the weight of the tumor were recorded to evaluate the tumor inhibition rate.The content of serum vascular endothelial growth factor （VEGF） in peripheral blood was analyzed by enzyme-linked immunosorbent assay （ELISA）.The micro-vessel density （MVD） of the tumor was assessed by immunohistochemistry（IHC）.The pulmonary metastasis was also recorded.[Results]As compared with the control group,the tumor weight decreased significantly in the Da-Huang-Zhe-Chong Pill group and the Bu-Yang-Huan-Wu Decoction group（P＜0.05）,while there was no significance in the Xue-Fu Zhu Yu Decoction group and the Gui-Zhi-Fu-Ling Pill group（P＞0.05）.The tumor inhibition rate were 41.76％,51.9％,2.14％,3.46％,respectively.As compared with the control group,the content of serum VEGF and the MVD increased significantly in the Da-Huang-Zhe-Chong Pill group（P＜0.05）,while there was no significance in the Bu-Yang-Huan-Wu Decoction group,the Xue-Fu-Zhu-Yu Decoction group,and the Gui-Zhi-Fu-Ling Pill group （P＞0.05）.As compared with the control group,the pulmonary metastasis increased significantly in the Da-Huang-Zhe-Chon

关 键 词：肝癌 瘤重 抑瘤率 血管内皮生长因子 微血管密度 肺转移 

分 类 号：R735.7[医药卫生—肿瘤]