转录因子Tbx18及Wt1在小鼠心脏发育过程中的时空表达  

Developmental patterns and characteristics of epicardial cell markers Tbx18 and Wt1 in murine embryonic hearts

作　　者：曾彬[1] 王艾力 彭小凡[3] 李昌 

机构地区：[1]武汉大学人民医院心血管内科,湖北武汉430062 [2]武汉大学中南医院心内科,湖北武汉430071 [3]武汉大学,临床医学08级2班湖北武汉430004 [4]湖北省中山医院心内科,湖北武汉430033

出　　处：《心脏杂志》2014年第2期133-137,共5页Chinese Heart Journal

基　　金：国家自然科学基金青年项目资助(30900609);国家自然科学基金面上项目资助(81270271)

摘　　要：目的：原位观察转录因子Tbx18及Wt1在胚胎心脏中的表达，及心肌细胞本身是否表达Tbx18及Wt1。方法：收集不同发育阶段小鼠胚胎（E）心脏，冰冻切片后，取不同区域的组织进行免疫荧光染色和DAPI染核，检测Tbx18、Wt1及Nkx2.5的表达。结果：小鼠前体心外膜及不同发育阶段的心外膜可明显表达Tbx18及Wt1蛋白，但未检测到心脏转录因子Nkx2.5的表达。从E10.5～至少E14.5d，Tbx18蛋白明显表达于不同区域的心脏组织中。除E14.5d少许表达Tbx18的细胞不表达Nkx2.5外，这些表达Tbx18的细胞还同时表达Nkx2.5。从E12.5～至少14.5d，Wt1表达于不同区域心脏组织中，但这些表达Wt1的细胞都不表达NKx2.5。结论：从E10.5～至少14.5d，Tbx18表达于小鼠心肌细胞中；从E12.5d～至少E14.5d，Wt1表达于小鼠心脏组织中，但不表达于心肌细胞中。AIM： To examine the expression of Tbx18 and Wtl in embryonic hearts and to explore whether Tbx18 and Wtl are expressed in the myocardium. METHODS： Mouse embryonic hearts were collected at different stages for immunofluorescence co-staining with either Tbx18 and the cardiac transcription factor Nkx2.5 or Wilms tumor 1 （Wt1） and Nkx2.5. RESULTS： Tbx18 and Wtl, but not Nkx2. 5, were expressed in the proepicardium and epicardium. Tbx18 was expressed in cells within the heart from El0.5 to at least El4. 5. These Tbx18-expressing cells were Nkx2.5 positive, except for a few cells that were Nkx2.5 negative at El4.5. Wtl was expressed in cells within the heart from El2.5 to at least E14.5, but these Wtl-expressing cells were Nkx2.5 negative. CONCLUSION ： The results of this study demonstrate that Tbx18 is expressed in the myocardium from E10.5 to at least E14.5 and Wtl is expressed within the heart from E12.5 to at least E14. 5, but not in the myocardium. These findings may provide some new insights on the role of epicardial cells in cardiac regeneration.

关 键 词：心脏干细胞 Tbx18 WT1 心脏发育 小鼠 

分 类 号：Q11[生物学—普通生物学]