miR101通过PLAP-1调节牙周膜细胞成骨分化的研究  

miR101 regulates the osteogenic differentiation of periodontal ligament cells via PLAP-1

作　　者：李春雷[1] 卢昌懿[2] 李长霞[3] 岳静[4,5] 黄欣[4,5] 吴补领[4,5] 

机构地区：[1]广东省人民医院口腔科,广东广州510080 [2]广州军区广州总医院护理部,广东广州510010 [3]广州军区联勤部门诊部,广东广州510080 [4]南方医科大学南方医院 [5]南方医科大学口腔医学院,广东广州510515

出　　处：《牙体牙髓牙周病学杂志》2014年第3期125-129,141,共6页Chinese Journal of Conservative Dentistry

基　　金：广东省自然科学基金(8451051501000371);广东省科技发展项目(2008B011300018-8);中国教育部研究生奖(20094433120004)

摘　　要：目的:研究miR101的表达变化靶向调节PLAP-1基因对牙周膜细胞成骨分化中细胞骨化功能的影响。方法:首先获得miR101过表达和表达抑制的慢病毒,以此转染人牙周膜细胞并对其进行成骨分化诱导,分别于矿化0、7、14、21 d采用定量RT-PCR检测各组靶基因PLAP-1mRNA的表达量;酶活性检测法检测各组细胞ALP活性变化;茜素红染色法检测各组细胞矿化结节形成情况。结果:miR101过表达后可抑制PLAP-1mRNA的表达,并使ALP活性升高、矿化结节形成量增多;而抑制miR101的表达后则可上调PLAP-1mRNA的表达水平,并使ALP的活性降低、矿化结节形成量减少。结论:在人牙周膜细胞骨向分化中miR101可能通过抑制PLAP-1而促进其成骨分化能力。AIM： To investigate the effects of miR-101 in the regulation of osteogenic differentiation of periodontal ligament ceils （PDLCs） through regulating target gene PLAP-1 expression. METHODS： HPLCs were transfected with miR101 and then inducted with osteogenic culture medium. At 0,7,14 and 21 d after transfection, RNA was extracted from HPLCs and PLAP-1 mRNA was measured by RT-PCR. ALP actively was determined by enzyme activity assay. Mineralized nodules were measured by alizarin red staining. RESULTS： miR101 overexpression inhibited PLAP - 1 mRNA expression, in creased ALP activity and mineralized nodule formation. On the contrary, inhibition of miR101 expression resulted in enhancement of PLAP-1 mRNA expression, decrease of ALP activity and mineralized nodule formation. CONCLUSION： miR101 may promote osteigenic differentiation of PDLCs through down - regulating target gene PLAP - 1 expression.

关 键 词：PLAP-1 牙周膜细胞 成骨分化 miR101 

分 类 号：R780.2[医药卫生—口腔医学]