CD^(4+)CD^(25+)T细胞在牙周炎发生时的免疫调节作用研究  

CD^(4+)CD^(25+)regulatory T cell proportion in the development of periodontitis

作　　者：杜毅[1] 于西佼[1] 唐开亮[1] 

机构地区：[1]济南市口腔医院牙体牙髓科,山东济南250001

出　　处：《牙体牙髓牙周病学杂志》2014年第3期142-145,共4页Chinese Journal of Conservative Dentistry

基　　金：山东省卫生科技攻关项目(2009HZ008)

摘　　要：目的:探讨CD4+CD25+Treg在牙周炎发生时的免疫调节作用。方法:取成年SPF级Wistar大鼠35只,用丝线结扎加高糖饮食法建立牙周炎动物模型;分别于术前(正常对照)以及结扎后第2、4、8、12周不同时间摄取各大鼠牙龈组织和外周血,用流式细胞仪检测CD4+CD25+Treg占CD4+T细胞的比例变化;用SPSS 13.0统计软件进行统计学分析,组间比较用独立样本t检验。结果:结扎后牙龈组中CD4+CD25+Treg占CD4+T细胞的比例(%)第2、4、8、12周各组分别为9.19±0.79、13.41±1.04、15.06±0.48、9.05±0.44,分别与正常对照组(6.69±1.22)相比P<0.05;2周与12周、4周与8周组间比较P>0.05,其他各组间比较P<0.05。外周血中CD4+CD25+Treg占CD4+T细胞的比例(%)在结扎后2、4、8、12周分别为6.34±1.32、8.02±1.35、9.12±0.24、9.34±0.56;各组间两两相比,结扎4、8、12周组明显高于正常对照组(5.90±0.71)(P<0.05),4、8、12周组间比较P>0.05。结论:CD4+CD25+Treg可能在牙周炎的发生、发展过程中起免疫调节作用。AIM： To observe CD^4＋ CD^25＋ regulatory T cell proportion in the development of periodontitis. METHODS ： 35 SPF Wistar rats were periodontally ligated and fed with high - sugar diet to induce periodontitis. Gingival tissues and peripheral blood were taken before and 2, 4, and 12 weeks after ligation. The proportion of CD^4＋ CD^25＋ Treg cells to CD^4＋ cells was measured by flow eytometry. RESULTS ： Before ligation （ control group） and 2, 4, 8 and 12 weeks after ligation the proportion（ % ） of CD^4＋ CD^25＋ T regulatory cells to CD^4＋ cells in gingival tissues was 6.69 ± 1.22, 9. 19 ± 0. 79, 13.41 ± 1.04, 15.06 ± 0.48 and 9. 05 ± 0. 44 respectively （ vs control group P 〈 0.05） ; that in peripheral blood was 5.90 ±0.71, 6.34 ± 1.32, 8.02± 1.35, 9. I2 ± 0.24 and 9.34 ± 0.56 respectively, between control and 4,8 or 12 - week, P 〈 0.05 ; between 4 and 8 or 12 - Week, P 〉 0.05. CONCLUSION： CD^4＋ CD^25＋ Treg cells may play regulatory roles in the progression of experimental periodontitis.

关 键 词：牙周炎 CD^4+ CD^25+ T细胞 免疫调节 

分 类 号：R780.2[医药卫生—口腔医学]