一氧化氮/诱导型一氧化氮合酶在动脉粥样硬化过程中的作用  

Effects of nitric oxide and inducible nitric oxide synthase on process of atherosclerosis

作　　者：边宁[1] 龚博君[1] 郭军[1] 黄正一[1] 李自成[1] 

机构地区：[1]暨南大学附属第一医院心血管内科,广东广州510630

出　　处：《中国病理生理杂志》2014年第3期414-418,共5页Chinese Journal of Pathophysiology

基　　金：广东省科技计划项目(No.20101096136);广州市科技计划(No.2010Y1-C941);暨南大学第一临床医学院重点学科基金(No.2010-4)

摘　　要：目的:探讨一氧化氮(NO)/诱导型一氧化氮合酶(iNOS)在动脉粥样硬化(atherosclerosis,AS)过程中的动态变化,分析其对动脉粥样硬化形成过程的影响。方法:将60只SD大鼠随机分成2组:对照组及AS组,每组30只。AS组采用维生素D3腹腔注射联合高脂饲料饲养的方法构建动脉粥样硬化模型。用相关生化方法检测血清各项生化指标:总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、空腹血糖和钙离子,比色法检测血清NO浓度,并对主动脉行HE染色,免疫组化技术检测iNOS蛋白表达,将所得数据进行统计分析,用简单线性相关分析NO与钙离子及动脉粥样硬化指数的相关性。结果:90 d后成功构建了主动脉中膜钙化型动脉粥样硬化模型。血清NO浓度在动脉粥样硬化过程中逐步下降,各组间差异均有统计学意义(均P<0.05)。动脉粥样硬化过程中动脉粥样硬化指数与钙离子呈正相关,与NO呈负相关。在90 d的AS组粥样斑块区免疫组化技术检测到iNOS蛋白表达。结论:在动脉粥样硬化形成过程中,主动脉粥样斑块区iNOS蛋白高表达,但血清NO浓度逐渐降低,NO抗动脉粥样硬化作用减弱。AIM ： To explore the dynamic changes of nitric oxide and inducible nitric oxide synthase during the process of atherosclerosis, and to analyze their influence on the formation of atherosclerosis. METHODS： SD rats （ n = 60） were randomly divided into control group and atherosclerosis group （30 rats in each group）. Atherosclerosis model was induced by feeding high-fat diet and vitamin D3. The values of blood biochemical were analyzed enzymatically using bioM6rieux kit. The concentration of serum nitric oxideing was detected by a colorimetric method. The success of athero sclerosis modeling was determined by pathological examination. The protein expression of inducible nitric oxide synthase in atherosclerotic plaque was detected by the method of immunohistochemistry. RESULTS： The atherosclerosis model was successfully established in 90 d. The concentration of serum nitric oxide gradually decreased in atherosclerosis group, and a significant difference among groups was observed. Atherosclerosis index was positively correlated with calcium ion, and negatively correlated with nitric oxide. The protein expression of inducible nitric oxide synthase in the atherosclerotic plaque after 90 d was found. CONCLUSION： The protein expression of inducible nitric oxide synthase in the plaque area of aorta increases and the concentration of serum nitric oxide decreases with the process of atherosclerosis. The anti-atherosclerosis role of nitric oxide is gradually decreased.

关 键 词：动脉粥样硬化 一氧化氮 一氧化氮合酶 钙 

分 类 号：R363[医药卫生—病理学]