纹状体立体定向注射Taclo建立帕金森病大鼠模型  

Establishment of PD rat model by injecting Taclo sterically and directionally into striatum

作　　者：孟祥继 庞琦[1] 丁锋[1] 辛涛[1] 杨洪安[1] 

机构地区：[1]山东大学附属省立医院神经外科,山东济南250021

出　　处：《山东大学学报（医学版）》2014年第3期16-18,22,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81100836;81171062)

摘　　要：目的观察大鼠纹状体立体定向注射1-三氯甲基-l,2,3,4-四氢化-β-咔啉(Taclo)对黑质内多巴胺神经元的损毁作用,研究帕金森病(PD)大鼠模型新型制作方法。方法将60只Wistar雄性大鼠随机分为聚乙二醇(PEG)组和Taclo组,每组30只。术后2周以阿扑吗啡诱导大鼠旋转,用自动视频追踪系统记录30 min内大鼠运动轨迹。术后1个月处死大鼠,行酪氨酸羟化酶(TH)免疫组化染色。结果阿扑吗啡诱导后,Taclo组大鼠表现为左侧恒定旋转,速度大于210 r/30 min,大鼠运动轨迹较稀疏,其右侧黑质多巴胺神经元数量比左侧及PEG组两侧明显减少(P<0.05)。结论纹状体立体定向注射Taclo可构建PD大鼠模型。Objective To observe the destructive effects of 1-three chlorine methyl-l,2,3,4-four hydrogenation of beta carboline （Taclo） on the dopaminergic neurons in the substantia nigra after striatal injection and search for a new method to establish parkinson disease （PD） rat model. Methods Sixty Wistar male rats were randomly divided into poly- ethylene glycol （PEG） and Taclo groups, 30 in each group. Apomorphine-induced rotation was observed two weeks after injection and rats＇ trajectory was recorded by the automatic video tracking system. The rats were sacrificed one month later and tyrosine hydroxylase （TH） immunohistochemistry was conducted. Results Twenty-four rats constant- ly rotated to the left side at a speed above 210 r/30 min in Taclo group. The trajectory became sparse in these rats. The results of immunohistochemistry showed that the number of TH-positive neurons in right substantia nigra of rats in Taclo group decreased than those in left substantia nigra and bilateral substantia nigra of rats in PEG group （ P 〈 0.05 ）. Twen- ty-four rats can be regarded as successful PD rat models. Conclusion PD rat model can be established by injecting Taclo sterically and directionally into the striatum.

关 键 词：帕金森病 大鼠动物模型 1三氯甲基l 2 3 4四氢化β咔啉 黑质多巴胺神经元 

分 类 号：R742.5[医药卫生—神经病学与精神病学]