EP4受体拮抗剂对雄激素非依赖性前列腺癌PC3细胞恶性表型的影响  

Effect of EP4 receptor antagonist on malignant phenotypes of androgen-independent prostate cancer cells PC3

作　　者：许松[1,2] 王立明[3] 葛京平[1,2] 周文泉[1,2] 张征宇[1,2] 

机构地区：[1]第二军医大学南京临床学院 [2]南京军区南京总医院泌尿外科,江苏省210002 [3]第二军医大学附属长征医院器官移植中心

出　　处：《江苏医药》2014年第5期497-500,F0002,共5页Jiangsu Medical Journal

基　　金：国家自然科学基金(81372742)

摘　　要：目的探讨EP4受体拮抗剂ONO-AE3-208对雄激素非依赖性前列腺癌(AIPC)PC3细胞的体外抗肿瘤效应。方法采用RT-PCR检测EP4受体mRNA在PC3细胞中的表达水平,实验组以0.1、1和10μmol/L的ONO-AE3-208作用于PC3细胞,对照组加入同体积的超轻水(ODW),采用MTT比色法、划痕愈合实验和细胞侵袭实验观察ONO-AE3-208对前列腺癌PC3细胞增殖、迁移和侵袭能力的抑制效应。结果 EP4受体在PC3细胞中呈高水平表达;随ONO-AE3-208作用时间和浓度的增加,其对PC3细胞的抑制率没有变化。PC3细胞的实验组细胞迁移比例为(25.30±6.11)%,低于对照组的(40.18±7.25)%(P<0.05)。实验组用0.1、1和10μmol/L的ONO-AE3-208作用于PC3细胞24h后,其穿入下室细胞数分别为(19.72±3.39)、(15.61±3.11)和(10.39±2.15)个/视野,少于对照组的(24.67±3.75)个/视野(P<0.05)。结论 EP4受体在AIPC PC3细胞中表达明显升高。EP4受体拮抗剂ONO-AE3-208对AIPC PC3细胞的增殖没有影响,但对其迁移和侵袭能力有明显的抑制作用。Objective To investigate the anti-tumor effect of EP4 receptor antagonist ONO- AE3-208 on androgen-independent prostate cancer（AIPC） cells PC3 in vitro. Methods Quantative RT-PCR was used to examine the EP4 mRNA expression in the PC3 cells. The influenee of ONO- AE3-208 on the proliferation of the cells was observed by MTT assay. Wound-healing assay and Transwell invasion assay were used to evaluate the inhibitory effect of ONO-AE3-208 on the ability of migration and invasion- Results EP4 was highly expressed in cell lines PC3. As the time and concentration inereased,EP4 antagonist ONO-AE3-208 did not influence the proliferation of the cells （P〉0. 05）. The migration distance ratio of ONO-AF.3-208 10 μmol/L in treatment group was （25. 30±6. ll）%,which was lower than （40. 18±7.25）% in control group（P〈0. 05）. After treated with ONO-AE3-208 0. 1,1.0 and10 μmol/L for 24 hours,the numbers of PC3 cells were （19.72±3. 39）, （15.61±3. 11） and （10.39±2.15） pieces/view,which were more than （24. 67±3.75） pieces/view in control group（P〈0. 05）. Conclusion AIPC PC3 cells highly express EP4. EP4 antagonist ONO-AE3- 208 does not effect the proliferation of AIPC PC3, but can suppress its ability of migration and invasion.

关 键 词：EP4受体 前列腺癌 

分 类 号：R737[医药卫生—肿瘤]