自组装多肽纳米支架对炎症刺激下成骨细胞迁移、增殖影响的研究  

Effect of Self-assembling Peptide Nano Stand on the Migration and Proliferation of Inflammatory Osteoblasts

作　　者：赵刚[1] 刘路阳[1] 蒋丽妍[1] 

机构地区：[1]佳木斯大学附属口腔医院正畸科,黑龙江佳木斯154007

出　　处：《口腔医学研究》2014年第3期197-200,共4页Journal of Oral Science Research

摘　　要：目的:通过改变氨基酸序列的方法,合成具有针对成骨细胞双重作用的多肽(RADA16-NBD),并检测其对炎症刺激下成骨细胞迁移、增值能力的影响。方法:制备RADA16-NBD水凝胶并用扫描电镜观察其结构。同时在多肽水凝胶支架RADA16-NBD上接种炎症刺激下的成骨细胞,观察细胞粘附、伸展、迁移和增殖的情况。结果:炎症刺激下成骨细胞能够在RADA16-NBD水凝胶中粘附、伸展、迁移和增值,并对炎症刺激下成骨细胞的迁移、增殖能力都有较高的表达。结论:自组装多肽水凝胶能够支持炎症刺激下成骨细胞的粘附、伸展、迁移和增殖,显示其既具有成骨又能抑制炎症活化的双重功能,为应用于炎症性骨吸收特别是牙周炎引起的骨缺损的治疗开拓一条新的方向和思路。Objective： To synthesize polypeptide（RADA16--NBD） which has dual functions on osteoblasts, and to detect its effect on the migration and proliferation of inflammatory osteoblasst by the method of changing the amino acid sequence. Methods： We prepare hydrogel RADA16--NBD and observe its structure by scanning electron mi- croscopy. At the same time we inoculate inflammatory osteoblasts on peptide hydrogel bracket RADA16-- NBD, and observe the adhesion, spreading, migration, and proliferation situation of cells. Results： Inflammatory osteo- blasts can adhere, spread, migration and proliferation in the RADA16--NBD hydrogel. Conclusions： Hydrogel has dual function in osteoblasts and inhibits the activation of inflammation, thus paves a new way in the treatment of in- flammatory bone resorption, especially in bone defects caused by periodontitis.

关 键 词：多肽纳米支架 RADA16-NBD 成骨细胞 组织工程 

分 类 号：R363[医药卫生—病理学]