牙龈蛋白酶诱导成骨细胞凋亡中促凋亡蛋白Bad的表达改变  

The expression of Bad in the process of gingipains-induced osteoblast apoptosis

作　　者：张福萍[1] 宋祥晨[1] 陈玉婷[1] 梁敏[1] 

机构地区：[1]中山大学光华口腔医学院.附属口腔医院,学士广东510055

出　　处：《中华老年口腔医学杂志》2013年第5期257-261,共5页Chinese Journal of Geriatric Dentistry

基　　金：国家自然科学基金(编号:81170970);留学归国人员科研启动基金(编号:2009134);广东省科技计划(编号:2011B031800259)

摘　　要：目的:检测牙龈蛋白酶诱导成骨细胞凋亡中Bad的表达改变,为保护成骨细胞提供依据。方法:8.348U/L牙龈蛋白酶处理成骨细胞0、4、8、16、24、48和72h,或将不同浓度牙龈蛋白酶与成骨细胞作用24h后,蛋白质印迹法检测Bad蛋白表达改变。结果:在一定范围内,牙龈蛋白酶在诱导成骨细胞凋亡中呈时间和剂量依赖性上调Bad的表达,牙龈蛋白酶抑制剂TLCK有效抑制牙龈蛋白酶所诱导的Bad的高表达。结论:牙龈蛋白酶上调Bad的表达,促凋亡蛋白Bad参与了牙龈蛋白酶诱导成骨细胞凋亡过程。Objective： To examine the expression of Bcl-xl/Bcl-2-Associated Death promoter（Bad） during osteoblast apop- tosis induced by gingipains. Methods： Mouse osteoblast cell line MC3T3-E1 cells were cultured in the presence of 8.348U/L gingipains for 0, 4, 8, 16, 24, 48 and 72h, or in different doses ofgingipains（0, 4.174, 8.348 and 16.696 U/L） for 24h. Protein expression of Bad was determined by Western blotting. Results： In this study, gingipains upregulated the expression of Bad in a time- and dose- dependent manner in osteoblast apoptosis. Proteinase inhibitor TLCK effectively inhibited increased of Bad expression induced by gingipains. Conclusions： Bad would play a role in the process of os- teoblast apoptosis induced by gingipains.

关 键 词：成骨细胞 细胞凋亡 BAD 牙龈蛋白酶 

分 类 号：R780.2[医药卫生—口腔医学]