筋骨草总黄酮治疗系膜增生性肾小球肾炎大鼠肾组织NF-κB变化的实验研究  

Experimental effects study of total flavonoids of Ajuga on nuclear transcriptional factor of kappa B in mesangial proliferative glomerulonephritis rats

作　　者：彭卫华[1,2,3,4,5] 南丽红[6] 赖文芳[6] 贾铷[6] 吴符火[6] 徐曾涛[6] 

机构地区：[1]南京军区福州总医院肾脏科,福州350025 [2]厦门大学附属东方医院 [3]福建医科大学 [4]福建中医药大学 [5]安徽医科大学福总临床医学院 [6]福建中医药大学药学院

出　　处：《中华临床医师杂志（电子版）》2013年第23期194-198,共5页Chinese Journal of Clinicians(Electronic Edition)

基　　金：福建省卫生厅中医药科研重点课题(wzzsb0906);陈可冀中西医结合发展基金.福建省中西医结合老年性疾病重点实验室开放课题资助项目(CKJ2008088);福建中医药大学校管课题(X2009069)

摘　　要：目的探讨筋骨草总黄酮(TFA)治疗系膜增生性肾小球肾炎(MsPGN)大鼠后核转录因子-κB(NF-κB)及下游转化生长因子-β1(TGF-β1)的变化。方法采用改良慢性血清病MsPGN大鼠模型,于造模第5周末检测尿蛋白,将尿蛋白阳性者随机分为模型组、雷公藤多甙(TWP)组、不同剂量TFA组。于造模第6周开始给药,6周后,测定各组大鼠的24 h尿蛋白定量、血生化和肾组织病理变化,MaxVisionTM即用型快速免疫组化一步法检测大鼠肾组织中NF-κB p65的表达,双抗体夹心法检测血清中TGF-β1的变化。结果 TFA可显著降低尿蛋白,明显减小系膜区面积占毛细血管丛面积的百分比(P<0.05或P<0.01);高、中剂量的TFA治疗效果与TWP相近(P>0.05)。模型对照组大鼠肾组织NF-κB p65、血清TGF-β1的表达明显高于正常对照组[NF-κB p65:28.93±4.45 vs.4.51±0.96,P<0.01;TGF-β1:(247.63±51.55)pg/ml vs.(127.52±27.84)pg/ml,P<0.01];干预性治疗后,TFA高、中剂量组、TWP组大鼠血清中NF-κB p65、TGF-β1的表达[NF-κB p65:10.35±2.81,15.21±4.71,11.70±3.97,P<0.01;TGF-β1:(181.96±52.93)pg/ml,(204.33±38.30)pg/ml,(188.07±42.92)pg/ml,P<0.05]较模型对照组明显降低。结论 TFA降低NF-κB并进而降低TGF-β1可能是治疗MsPGN大鼠的作用机制之一。Objective To investigate the effects of total flavonoids of Ajuga （TFA） on nuclear transcriptional factor of kappa B （NF-κB） in mesangial proliferative glomerulonephritis （MsPGN） rats. Methods MsPGN rat was induced by the improved chronic serum sickness model after five weeks. the positive urinary protein rats were randomly divided into model group, triptergium wilfordii polyglycosidium （TWP） group and TFA group with different dosage, normal rats was considered as the control group. MsPGN rats were treated with drugs since the sixth week; succeeding six weeks later,all rats were detected 24 h urinary protein, blood biochemistry, NF-κB change in renal tissue by immunohistochemical method, serum transforming growth factor beta 1 （TGF-β1） was measured by double antibody sandwich ELISA immunoassay. Results Compared with model group, TFA can significantly reduce the urinary protein, decrease BUN, Scr, TG, TC, mitigate the morphological damage （P＆lt;0.05 or P＆lt;0.01）;The effects of high-dosage （2.160 g/kg） TFA and middle-dosage （1.080 g/kg） TFA were as similar as those of TWP （P＆gt;0.05）. The renal tissue NF-κB （Integral optical density, IOD） and serum TGF-β1 （pg/ml） in model control was significantly higher than that in normal control group, NF-κB p65 28.93±4.45 vs. 4.51±0.96, P＆lt;0.01; TGF-β1： （247.63±51.55）pg/ml vs. （127.52±27.84）pg/ml, P＆lt;0.01; With the intervention trerapy of high-dosage TFA, middle-dosage TFA and TWP, the expression of NF-κB in renal tissue and TGF-β1 in serum was significantly decreased compared with model control group, NF-κB p65： 10.35±2.81, 15.21±4.71,11.70±3.97, P＆lt;0.01; TGF-β1： （181.96±52.93）pg/ml, （204.33±38.30）pg/ml, （188.07±42.92）pg/ml, P＆lt;0.05. Conclusion Reducing the expression of NF-κB and successive TGF-β1 may be one of the mechanisms of total flavonoids of Ajuga treatment on MsPGN rats.

关 键 词：筋骨草属 肾小球肾炎 膜增生性 转化生长因子β1 TRANSFORMING growth factor BETA1 

分 类 号：R285.5[医药卫生—中药学]