慢性高原病骨髓单个核细胞凋亡及Fas、FasL、TNFR和TNF表达研究  

Studies on expressions of Fas, FasL, TNFR and TNF and apoptosis of bone marrow mononuclear cells in chronic mountain sickness

作　　者：王传玲[1] 崔森[2] 李占全[2] 冀林华[2] 柴克霞[2] 

机构地区：[1]青海大学研究生院,西宁810001 [2]青海大学附属医院血液科

出　　处：《中华临床医师杂志（电子版）》2013年第24期109-111,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：中国科学院"西部之光"人才培养计划资助项目[中国科学院"科发人教字(2002)404号"文件];青海省科技创新能力促进计划(2010-Z-723);国家自然科学基金委员会地区科学基金(81260300)

摘　　要：目的慢性高原病(CMS)是高海拔地区常见病。本文通过研究CMS患者骨髓单个核细胞(BMMNCs)凋亡及Fas、FasL、TNFR和TNF表达情况,探讨细胞凋亡及凋亡分子在CMS发病中的作用。方法选取CMS患者35例,采用电镜定性观察骨髓造血细胞凋亡情况,TUNEL技术定量研究BMMNCs凋亡指数,流式细胞术检测BMMNCs凋亡分子Fas、FasL、TNFR及TNF表达水平,并与健康对照组(20例)比较。结果 (1)CMS患者骨髓造血细胞存在凋亡现象,CMS患者BMMNCs凋亡指数明显低于对照组,分别为(10.13±9.80)%及(21.32±11.97)%(P<0.01);(2)BMMNCs凋亡分子Fas、FasL、TNFR及TNF表达水平在两组间无统计学差异。结论 CMS患者BMMNCs凋亡下调,可能在CMS红细胞积累中发挥一定作用,但其凋亡异常的机制比较复杂,可能涉及多种凋亡信号转导途径。Objective To observe the expressions of Fas, FasL, TNFR and TNF and the change of apoptosis of bone marrow mononuclear cells （BMMNCs） in patients with chronic mountain sickness（CMS）. Methods The bone marrow specimens were collected from 35 CMS patients and 20 healthy adults and the BMMNCs were separated. Apoptosis was observed qualitatively by electron microscopy. The apoptotic index （AI） of BMMNCs was measured by terminal deoxynucleotidyl transferase-mediated nick end labeling （TUNEL） technique. Flow cytometry was used to detect expressions of Fas, FasL, TNFR and TNF of BMMNCs. Results （1）There was apoptosis of BMMNCs in patients with CMS. The AI of BMMNCs in CMS patients was lower than that in the controls （10.13%±9.80 % vs. 21.32%±11.97%, P〈0.01）. （2） The significant difference was not found between the two groups in the expression of Fas, FasL, TNFR or TNF. Conclusions The results showed that apoptosis of BMMNCs was down-regulated in CMS patients, which may play a role in the accumulation of red blood cells. The mechanism of change of hematopoietic cells apoptosis in CMS patients is complicated, other apoptotic signal transduction pathways may be involved in it.

关 键 词：高原病 造血干细胞 细胞凋亡 抗原 CD95 受体 肿瘤坏死因子 FASL TNFR 

分 类 号：R594.3[医药卫生—内科学]