Surface patterning and modification of polyurethane biomaterials using silsesquioxane-gelatin additives for improved endothelial affinity  

作　　者：HOU LinXi PECK Yvonne WANG XiaoWen WANG DongAn 

机构地区：[1]College of Chemistry and Chemical Engineering, Fuzhou University [2]Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University

出　　处：《Science China Chemistry》2014年第4期596-604,共9页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21376054);the Educational Commission of Zhejiang Province of China(Y201223742);the AcRF Tier 1 Grant RG 36/12,Ministry of Education,Singapore

摘　　要：Polyurethanes(PUs) are well-known for their biocompatibility but their intrinsic inert property hampers cell-matrix interactions. Surface modifications are thus necessary to widen their use for biomedical applications. In this work, surface modifications of PU were achieved first by incorporating polyhedral oligomeric silsesquioxane(POSS), followed by alteration of the surface topography via the breath figures method. Subsequently, surface chemistry was also modified by immobilization of gelatin molecules through grafting, for the enhancement of the surface cytocompatibility. Scanning electron microscopy(SEM) was used to verify the formation of highly ordered microstructures while static contact angle, FTIR and XPS confirmed the successful grafting of gelatin molecules onto the surfaces. In vitro culture of human umbilical vein endothelial cells(HUVECs) revealed that endothelial cell adhesion and proliferation were significantly enhanced on the gelatin-modified surfaces, as shown by live/dead staining and WST-1 proliferation assay. The results indicated that the combination of the strategies yielded an interface that improves cell attachment and subsequent growth. This enhancement is important for the development of higher quality biomedical implants such as vascular grafts.Polyurethanes （PUs） are well-known for their biocompatibility but their intrinsic inert property hampers cell-matrix interactions. Surface modifications are thus necessary to widen their use for biomedical applications. In this work, surface modifica- tions of PU were achieved first by incorporating polyhedral oligomeric silsesquioxane （POSS）, followed by alteration of the surface topography via the breath figures method. Subsequently, surface chemistry was also modified by immobilization of gelatin molecules through grafting, for the enhancement of the surface cytocompatibility. Scanning electron microscopy （SEM） was used to verify the formation of highly ordered microstructures while static contact angle, FTIR and XPS confirmed the successful grafting of gelatin molecules onto the surfaces. In vitro culture of human umbilical vein endothelial cells （HUVECs） revealed that endothelial cell adhesion and proliferation were significantly enhanced on the gelatin-modified surfaces, as shown by live/dead staining and WST-1 proliferation assay. The results indicated that the combination of the strategies yielded an interface that improves cell attachment and subsequent growth. This enhancement is important for the development of higher quality biomedical implants such as vascular grafts.

关 键 词：BIOMATERIALS surface modification pattern POLYURETHANE SILSESQUIOXANE GELATIN endothelization 

分 类 号：TQ323.8[化学工程—合成树脂塑料工业]