Preparation of stable micropatterns of gold on cell-adhesion-resistant hydrogels assisted by a hetero-bifunctional macromonomer linker  

作　　者：SUN JianGuo GRAETER Stefan V TANG Jian HUANG JingHuan LIU Peng LAI YuXiao YU Lin MAJER Günter SPATZ Joachim P DING JianDong 

机构地区：[1]State Key Laboratory of Molecular Engineering of Polymers [2]Department of Macromolecular Science, Fudan University [3]Research Centre, Eye and ENT Hospital, Fudan University [4]Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems [5]Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg

出　　处：《Science China Chemistry》2014年第4期645-653,共9页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21034002,91127028,51273046);the Ministry of Science and Technology of China(2011CB606203);the Science and Technology Development Foundation of Shanghai(13XD1401000)

摘　　要：Surface patterning is very useful in biomaterial studies, yet it is not easy to prepare a micropattern with cell-adhesion contrast that is stable in a wet environment. Recently, a platform technique of transfer photolithography was invented to fabricate stable metal microarrays on the surface of a cell-adhesion resistant and mechanically biomimetic poly（ethylene glycol） hydrogel; the linker is the key chemical in such a transfer strategy. This article reports the design and synthesis of a hetero-bifunctional macromonomer linker with a thiol group at one end and an acryloyl group at the other end. The bifunctional linker was char- acterized by GPC and ~H NMR, and the average number of thiol groups in the bifunctional linker was detected by Ellman＇s reagent. The regent stability under wet conditions was also confirmed by the model reactants. The resultant micropatterned surfaces are meaningful for future studies of cell behaviors on mechanically biomimetic matrixes.Surface patterning is very useful in biomaterial studies, yet it is not easy to prepare a micropattern with cell-adhesion contrast that is stable in a wet environment. Recently, a platform technique of transfer photolithography was invented to fabricate stable metal microarrays on the surface of a cell-adhesion resistant and mechanically biomimetic poly(ethylene glycol) hydrogel; the linker is the key chemical in such a transfer strategy. This article reports the design and synthesis of a hetero-bifunctional macromonomer linker with a thiol group at one end and an acryloyl group at the other end. The bifunctional linker was characterized by GPC and 1H NMR, and the average number of thiol groups in the bifunctional linker was detected by Ellman's reagent. The regent stability under wet conditions was also confirmed by the model reactants. The resultant micropatterned surfaces are meaningful for future studies of cell behaviors on mechanically biomimetic matrixes.

关 键 词：biomaterials HYDROGELS MICROPATTERN MACROMONOMER cell adhesion 

分 类 号：O648.17[理学—物理化学] TB383[理学—化学]