四种基因在单发和多发性膀胱癌组织中的表达及意义  

Expression and significance of p16,p21,p53 and EMA in single and multiple bladder cancer

作　　者：刘大刚[1] 王丽琼[1] 朱惠荣[1] 杨芳[1] 

机构地区：[1]昆明医科大学附属延安医院病理科,昆明650051

出　　处：《中国肿瘤临床与康复》2014年第3期289-292,共4页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的探讨p16、p21、p53和上皮细胞膜抗原(EMA)在单发性和多发性膀胱癌组织中的表达及意义。方法收集临床正常、单发和多发性膀胱癌标本,通过免疫组化法检测膀胱癌组织中相关基因的蛋白表达和定位分布。结果与正常膀胱黏膜上皮相比,p16和p53蛋白定位于细胞核,随着膀胱癌恶性程度的升高和侵袭程度的增加,p16蛋白表达明显下降,而p53表达明显升高(P<0.05)。p21蛋白定位于细胞膜,高分化膀胱癌的p21蛋白表达低于低分化膀胱癌(P<0.05);多发性膀胱癌表达阳性率明显高于单发性(P<0.05)。EMA主要表达于细胞浆,随着肿瘤分级和(或)分期的增加,阳性细胞数逐渐减少(P<0.05),在单发和多发性膀胱癌中的表达差异有统计学意义(P<0.05)。结论 p16、p53、p21和EMA在膀胱癌组织中不同水平的表达与膀胱癌的分级/分期密切相关,对膀胱癌相关基因的检测有助于膀胱癌的临床诊断、预后判断和治疗方法的选择。Objective To explore the expression and implicaitons of p16, p21, p53 and EMA in single and multiple bladder eance. Methods Samples of normal bladder, single and multiple bladder canc- er were collected,and the expression and locational distribution of p16, p21, p53 and EMA in these tissues were tested through immuno-bistoehemical technique. Results In contrast to normal bladder mucous mem- brane ,p16 and p53 mainly stayd in cell nucleus. With malignancy and infiltrating of bladder cancer increas- ing,the expression of p16 descended, while the expression of p53 ascended distinctly （P 〈 0. 05 ）. p21 mainly stayed in cytoplasm ,the expression of p21 in well-differentiated bladder cancer was lower than poorly differentiated bladder cancer （ P 〈 0.05 ） , the positive rate of multiple bladder cancer was higher than that of single bladder cancer （ P 〈 0. 05 ）. EMA stayed mainly in cytomembrane. With the grades and stages of tumors increasing, the positive cells decreased gradually （ P 〈 0. 05 ）. The expression of EMA was signifi- cantly different between single and multiple bladder cancer （ P 〈 0. 05 ）. Conclusions The different level expression of p16, p21, p53 and EMA in bladder cancer is closely related to the grades and stages of bladder cancer;Test of the relative gene of bladder cancer is helpful to clinical diagnosis, prognosis judgement and treatment selection.

关 键 词：膀胱肿瘤 基因 p16 基因 p21 基因 p53 上皮细胞膜抗原 免疫组化 

分 类 号：R737.14[医药卫生—肿瘤]