胃癌组织Wnt10b的表达及意义  

Expression of Wnt10b in gastric cancer tissue and its significance

作　　者：王林[1,2] 许晓群[1] 王郡甫[1] 

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,山东济南250062 [2]济宁医学院附属济宁市第一人民医院,山东济宁272011

出　　处：《济宁医学院学报》2014年第1期30-32,36,共4页Journal of Jining Medical University

基　　金：山东省自然科学基金(ZR2009CM036;ZR2010CM067);山东省大型科学仪器设备升级改造技术研究专项(2013SJGZ09)

摘　　要：目的检测胃癌及癌旁组织中Wnt10b的表达,探讨其与临床病理参数的关系。方法收集25例胃癌患者手术切除的癌组织及对应的癌旁组织,用实时荧光定量PCR检测Wnt10bmRNA的表达,Westernblot技术检测Wnt10b蛋白的表达,分析Wnt10bmRNA在胃癌组织中的表达与临床病理参数的关系。结果胃癌组织中Wnt10bmRNA和蛋白表达水平明显高于癌旁组织(P<0.001),胃癌组织中Wnt10bmRNA表达阳性率与肿瘤远处转移相关(P<0.05),但与年龄、性别、肿瘤大小、TNM分期、分化程度和浸润深度无关。结论Wnt10b在胃癌组织中呈活化状态,可能参与胃癌的发生发展。Objective This study aimed to detect the expression of Wnt10b in paired tumor and non-cancerous tissues of gastric cancer （GC）patients and explore correlation of Wnt10b mRNA expression with the clinicopatho-logical parameters of GC.Methods The surgically resected paired cancerous and adjacent non-cancerous tissues of 25 GC patients were collected.The expression of Wnt10b mRNA was examined by Quantitative real-time PCR （qRT-PCR）.Western-blot was employed to detect Wnt10b protein levels.Correlation between Wnt10b mRNA ex-pression and clinicopathological parameters was analyzed.Results The levels of Wnt10b mRNA in cancerous tis-sues were higher compared with matching adjacent non-cancerous tissues （P〈0.001 ）.The protein levels of Wnt10b were consistent with the expression levels of Wnt10b mRNA.Wnt10b mRNA expression was correlated with the lymph node metastasis of tumor （P〈0.05）,but no association was found between Wnt10b mRNA expres-sion and other clinicopathological parameters including age,gender,tumor size,TNM stage,differentitation stage and depth of invasion.Conclusion The expression levels of Wnt10b in cancerous tissues were higher than adjacent non-cancerous tissues,and there was correlation between the expression levels of Wnt10b and the lymph node me-tastasis of tumor.

关 键 词：胃癌 Wnt10b 实时荧光定量PCR WESTERN-BLOT 

分 类 号：R735.2[医药卫生—肿瘤]