适于百合遗传多样性分析的RAPD引物筛选  

RAPD Primers Screening Suitable for Genetic Diversity Analysis in Lilium

作　　者：陈名红[1,2] 刘多[1] 熊华斌[1] 杜刚[1] 熊勇[1] 李成云[2] 

机构地区：[1]云南民族大学民族药资源化学国家民委-教育部重点实验室,昆明650031 [2]云南农业大学农业生物多样性与病虫害控制教育部重点实验室,昆明650201

出　　处：《种子》2014年第3期1-3,共3页Seed

基　　金：国家自然科学基金(编号:30860161);云南省教育厅科学研究基金(编号:2013 Z 040)

摘　　要：为筛选出适于百合遗传多样性分析的RAPD有效引物,利用48个RAPD随机引物,对百合属的黄天霸、西伯利亚、兰州百合和川百合进行PCR扩增,共筛选出8条多态性丰富、条带清晰且可重复性好的有效引物,8条引物在4个样品中共扩增出112条DNA带,其中78条为多态性带,占总扩增带数的69.6%,平均每个引物扩增出14条带。该研究为应用RAPD标记技术进行百合属植物遗传多样性分析和品种分子鉴别等研究奠定了基础。The aim was to provide effective primers suitable for genetic diversity analysis in Lilium with RAPD markers.48 RAPD primers were used in the RAPD-PCR amplification for Manissa,Siberia,L.davidii var.unicolor and L.davidii,and 8 effective primers with characteristics of rich polymorphism,clear bands and good repeatability were screened.112 DNA bands were obtained,of which 78 bands were polymorphic,accounting for 69.6％ of the total amplified bands.And 14 bands could be amplified with a primer,averagely.The research results will lay the foundation for the study on genetic diversity analysis,cultivar identification of Lilium at the molecular level.

关 键 词：百合属 RAPD标记 引物 筛选 

分 类 号：S644.1[农业科学—蔬菜学]