糖皮质激素诱导的亮氨酸拉链蛋白、Y-盒结合蛋白1和P-糖蛋白在上皮性卵巢癌组织中的表达及其与化疗耐药的关系  

The expression of GILZ,YB-1 and P-gp in epithelial ovarian cancer and its relationship to drug resistance

作　　者：谭爽[1,2] 常苗苗[1] 邹忠文[1] 

机构地区：[1]大连医科大学附属第二医院妇产科,辽宁大连116027 [2]大连医科大学,辽宁大连116023

出　　处：《中国实用妇科与产科杂志》2014年第4期297-300,共4页Chinese Journal of Practical Gynecology and Obstetrics

摘　　要：目的探讨上皮性卵巢癌中糖皮质激素诱导的亮氨酸拉链蛋白（GILZ）、Y-盒结合蛋白1（YB-1）和P-糖蛋白（P—gP）的蛋白表达，分析其与卵巢癌化疗耐药的关系。方法2005年3月至2012年2月在大连医科大学第-、第二附属医院采用免疫组织化学sP法检测85例上皮性卵巢癌，30例卵巢良性肿瘤和12例正常卵巢组织中GILZ、YB-1和PGP的蛋白表达情况，并结合临床病理资料进行综合分析。结果GILZ、YB-1和P—gP在上皮性卵巢癌中表达阳性率分别为51．8％、48．2％和47．1％，在卵巢良性肿瘤和正常卵巢组织中均未见表达，差异有统计学意义（P〈0．01）。YB-1和P—gP在Ⅲ-Ⅳ期上皮性卵巢癌中的表达高于Ⅰ～Ⅱ上皮性卵巢癌中的表达（P〈0．05）。GILZ、YB-1和P—gP三者相关，GILz与YB-1，r=0．344；P—gP与GILZ，r=0．437；P—gp与YB-1，r=0．570（P均〈0．01）。44例GILZ蛋白阳性表达者中18例为化疗耐药，41例GILZ蛋白阴性表达者中6例为化疗耐药，差异有统计学意义（P〈0．05）。41例YB-1蛋白阳性表达者中17例为化疗耐药，44例YB—1蛋白阴性表达者中7例为化疗耐药，差异有统计学意义（P〈0．05）。40例P—gP蛋白阳性表达者中17例为化疗耐药，45例P—gP蛋白阴性表达者中7例为化疗耐药，差异有统计学意义（P〈0．05）。结论GILZ、YB-1和P—gP可能参与了卵巢癌的发生、发展过程。3种蛋白在卵巢癌化疗耐药中发挥-定作用，独立或联合检测可能预测化疗的敏感性。Objective To study the expression of GILZ, YB-1 and P-gp in epithelial ovarian cancer （EOC） and its rela- tionship to drug resistance. Methods The expression of GILZ, YB-1 and P-gp proteins in 85 cases of epithelial ovarian cancer ,30 cases of epithelial ovarian benign tumor and 12 cases of normal ovarian was detected by immunohistochemistry, and clinical pathological features was analyzed. Results The positiveexpression rate of GILZ, YB-1 and P-gp in epitheli- al ovarian cancer was 51.8% ,48. 2% and 47.1% respectively,which was significantly higher than that in epithelial be- nign ovarian tumor（negative） and normal ovarian（negative） （P 〈 0. 01 ）. The expression rate of YB-1 and P-gp was higher in clinical Ⅲ and Ⅳ phases than in I and II phases （ P 〈 0. 05 ）. The relation Of GIIZ, YB-1 and P-gp was notably signif- icant（ GILZ vs. YB-1, r -- 0. 344, P-gp vs. GILZ, r = 0. 437, P-gp vs. YB-1, r = 0. 570, P 〈 0.01 ）. The drug resistance rate in positive expression tissues of GILZ was higher than in negative expression tissues （P 〈 0.05 ）. The drug resistance rate in positive expression tissues of YB-1 was higher than in negative expression tissues （P 〈 0.05 ）. The drug resistance rate in positive expression tissues of P-gp was higher than in negative expression tissues （P 〈 0. 05）. Conclusion GILZ, YB-1 and P-gp may play a significant role in the growth and drug resistance of epithelial ovarian cancer. Detections of GILZ,YB-1 and P-gp may guide the using of chemotherapy drugs of ovarian cancer and decrease drug resistance.

关 键 词：卵巢肿瘤 糖皮质激素诱导的亮氨酸拉链蛋白 Y-盒结合蛋白1 P-糖蛋白 化疗耐药 

分 类 号：R737.3[医药卫生—肿瘤]