双波长薄层扫描法测定不同来源枸杞子中甜菜碱的含量  

Determination of Betaine Contents in Fructus Lycii from Different Origins by Dual Wavelength TLC Scanning

作　　者：谭亮[1] 冀恬[1] 曹静亚[1] 胡风祖[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810008

出　　处：《天然产物研究与开发》2014年第3期388-391,397,共5页Natural Product Research and Development

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-EW-J-26)

摘　　要：通过对枸杞子样品提取、脱色时间等前处理条件的优化，建立不同来源枸杞子中甜菜碱含量测定的双波长薄层扫描法（TLCS法）。使用快速溶剂萃取仪（ASE350）用80％甲醇提取出枸杞子中的甜菜碱，经活性炭脱色、雷氏盐沉淀、丙酮溶解沉淀，采用改进的薄层扫描法在检测波长为530nm，参比波长为625nm条件下对枸杞子中的甜菜碱进行含量测定。得到清晰的薄层色谱斑点，无干扰；甜菜碱点样量在3．84—38．40ug范围内线性关系良好，r=0．9995；平均加样回收率为98．30％，RSD=2．55％（n=9）。该方法简便、准确，重现性好，适用于测定枸杞子中甜菜碱的含量测定，可为枸杞的质量控制提供依据。In this study, a thin layer chromatography scanning （TLCS） method was developed for the determination of betaine contents in Fructus Lycii from different origins. Betaine was extracted using accelerated solvent extractor （ ASE 350） with 80% aqueous methanol. The detection and reference wavelengths of the developed TLCS method were 530 nm and 625 nm after activated charcoal decolorize,redwood salt deposit and acetone dissolve. TLC spots were clear and no interference was appeared. The linear response ranges of betaine was 3.84 - 38.40 ug （r = 0.9995） ;The average recoveries （n = 9） were 98.30% ,RSD = 2, 55%. The assay demonstrated that the developed method was accurate. It was suitable for the determination of betaine in F. Lycii. In addition,it can be used to assist the quality evaluation of Lycium barbarum L.

关 键 词：双波长薄层扫描法 枸杞子 甜菜碱 不同来源 含量测定 

分 类 号：R284.2[医药卫生—中药学]