肾茶提取物抗氧化及保护线粒体作用研究(英文)  

In vitro Antioxidant and Mitochondria Protective Activities of Clerodendranthus spicatus Extracts

作　　者：陈地灵[1] 龙贺明[2] 张鹤鸣[1] 李宁[1] 刘颂豪[1] 

机构地区：[1]华南师范大学药物研究院,广州510631 [2]赣南医学院第一附属医院肿瘤科,赣州341000

出　　处：《天然产物研究与开发》2014年第3期392-397,共6页Natural Product Research and Development

摘　　要：肾茶水提液依次用石油醚、氯仿、乙酸乙酯萃取,与水层得到4个不同极性部位。以清除DPPH·、超氧阴离子和羟自由基的能力,络合Fe2+能力,体外抗活性氧能力以及对FeSO4和H2O2诱导小鼠肾脏组织匀浆脂质过氧化产物MDA和GSH-Px水平的影响,综合评价其抗氧化活性;以抑制Fe2+-L-Cys诱导肾脏线粒体肿胀能力,初步评价其保护肾脏线粒体的作用。结果显示肾茶水提液4个不同极性部位均具有不同程度的清除DPPH·、O-·2、·OH和络合Fe2+能力;其中乙酸乙酯和水提部位具有显著体外抗ROS能力,降低MDA和升高GSHPx水平,以及抑制肾脏线粒体肿胀的能力,且其作用效果均与剂量呈正相关。本实验结果可为肾茶用于治疗肾脏疾病的药效物质基础研究提供依据。Water extract of Clerodendranthus spicatus was successively fractionated using 3 different solvents to provide petroleum ether extract （PE）, chloroform extract （CE）, ethyl acetate extract （EAE） and water extract （WE）. Different antioxidant assays including OH · , O2- , DPPH · scavenging activity, reducing power, metal chelating activity, anti-reactive oxygen species （anti-ROS） activity and effects on FeSO4 and H2O2 induced MDA and GSH-Px production increase were employed to evaluate the antioxidant activities of four extracts. The inhibition rate of mitochondrial swelling induced by Fe2 ＋ -L-Cys in vitro was employed to evaluate the protective effects on mitochondrion. The results showed that all the 4 different extracts had the ability of scavenging DPPH · , O2- , · OH and chelating Fe2＋ , anti-ROS in vitro, significantly lower the levels of MDA,rised the levels of GSH-Px in renal homogenate and inhibited the renal mitochondria swelling, in concentration dependent manner. The results provided a basis for C. spicatus to be used in the treatment of renal disease.

关 键 词：肾茶 抗氧化活性 金属络合能力 活性氧 线粒体肿胀 

分 类 号：R285.5[医药卫生—中药学]