六种花提取物抑制α-葡萄糖苷酶活性研究  

α-Glucosidase Inhibitory Activity of Extracts from Six Kinds of Flowers

作　　者：常美芳[1] 施余杰[1] 顾海鹏[1] 尹震花[1] 康文艺[1] 

机构地区：[1]河南大学中药研究所,开封475004

出　　处：《天然产物研究与开发》2014年第3期410-413,437,共5页Natural Product Research and Development

基　　金：河南省科技厅重点攻关项目(102102310019;12210231 0170);河南省科技厅基础前沿计划(112300410075);河南省青年骨干教师计划(教高2012-704)

摘　　要：采用96微孔板法对紫丁香、日本晚樱、锦带花、荆条花、疏毛绣线菊和猥实中不同提取部位对α-葡萄糖苷酶抑制活性进行评价,并与阳性对照药阿卡波糖进行了比较,发现6种花不同提取部位均具有一定的α-葡萄糖苷酶抑制活性。其中,荆条花的乙酸乙酯部位(IC50=290.57μg/mL)和日本晚樱的乙酸乙酯部位(IC50=292.72μg/mL)抑制活性最好,且都远大于阳性对照阿卡波糖(IC50=1213.38μg/mL)。不同溶剂提取物显示,紫丁香、日本晚樱、锦带花和荆条花的乙酸乙酯部位抑制活性均大于正丁醇部位;而疏毛绣线菊和猥实的正丁醇部位略大于乙酸乙酯部位,且6种花的石油醚部位活性最低。此外,各部位的抑制活性均与质量浓度呈现相关性,具有一定的浓度依赖性。a-Glucosidase inhibitory activity of different extracts from Syringa oblata Lindl. , Prunus serrulata var. lannesi- ana （ Carr. ） Rehd. , Weigela florida （Bunge） A. DC. , Vitex negundo L. var. heterophylla （ Franeh. ） Rehd. , Spiraea hirsuta （Hemsl.） Sehneid. ,Kolkwitzia amabilis Graebn. ） was evaluated compared with aearbose as positive control in vitro. The result showed that extracts from six kinds of flowers all had a-glueosidase inhibitory activity, which V. negundo ethyl acetate extract （IC50 = 290.57 ug/mL） and P. serrulata var. lannesiana ethyl acetate extract （IC50 = 292.72 ug/ mL） had the highest activity. They were all higher than that of the positive control acarbose （IC50 = 1213.38 ug/mL）. Among different extracts, inhibitory activity of ethyl acetate extract of S. oblata, P. serrulata var. lannesiana, W. florida and V. negundo were higher than that of n-butanol extract,while n-butanol extract of S. hirsuta and IC amabilis was higher than that of their ethyl acetate extract, and inhibitory activity of petroleum ether extract was the lowest. In addition, the concentration of all extracts showed positive correlation with inhibitory activity, and also showed a certain degree of concentration dependence.

关 键 词：荆条花 日本晚樱 a-葡萄糖苷酶 抑制活性 

分 类 号：R284.2[医药卫生—中药学]