血卟啉单甲醚介导的声动力对U937细胞增殖影响  

The sonodynamic effect of HMME on the proliferation of U937 cells

作　　者：王元[1] 苏晓敏[1] 刘晶 张婷[1] 王筱冰[1] 刘全宏[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062 [2]西安630医院检验科,陕西西安710000

出　　处：《陕西师范大学学报（自然科学版）》2014年第2期77-81,共5页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(81000999);陕西师范大学大学生创新科研项目(CX13073)

摘　　要：研究了聚焦超声激活血卟啉单甲醚对人髓系白血病细胞株U937细胞的增殖抑制作用.通过MTT法检测了频率为1.1 MHz的聚焦超声激活血卟啉单甲醚作用于U937肿瘤细胞的存活率.MTT实验结果显示：声照辐射60 s、强度为2～7 W/cm2的超声对U937细胞的抑制作用呈强度依赖性;单纯血卟啉单甲醚组与对照组相比无显著性差异（P＞0.05）;同等条件下,2 W/cm2超声结合10 ng/L血卟啉单甲醚组对U937肿瘤细胞有明显的协同杀伤作用,与对照组相比有极显著性差异（P＜0.01）.这些结果表明,超声联合血卟啉单甲醚声动力能够特异性抑制U937细胞生长,血卟啉单甲醚有望成为一种新型声敏剂应用于白血病的声动力治疗.The present study aims to investigate the cytotoxic effect of focused ultrasound combining HMME on human leukemia U937 cells.Tumor cells suspended in serum-free RPM1640 were exposed to ultrasound with 1 W/cm2 and 1.1 MHz for 60 seconds in the presence and absence of HMME.The viability of cells was determined by MTT.MTT results showed that the treatment with ultrasound （1.1 MHz,2～7 W /cm2,60 s） alone significantly inhibited the cell proliferation of the U937 cells in a intensity dependence,while the HMME （10～ 100 ng/L） alone showed no statistically significant differences with the control groups （P〉0.05）; Under the same conditions,the synergistic effect of 2 W/cm2 ultrasound combined with HMME showed significant cell killing compared with control （P 〈 0.01）.These results demonstrated that ultrasound combined with HMME exerted specific inhibitory effect on the proliferation of U937 cells.HMME may be a promising sonosensitizing agent for the treatment of human leukemia.

关 键 词：超声 血卟啉单甲醚 U937细胞 

分 类 号：Q274[生物学—细胞生物学]