原发性肝细胞癌患者Galectin-3和Galectin-9的表达及其与预后的关系  

Expressions of Galectin-3 and Galectin-9 in hepatocellular carcinoma patients and their relationship with prognosis

作　　者：孔菲[1] 马洪喜[2] 姜晶[1] 宋莹[1] 郭长松[1] 金美善[2] 牛俊奇[3] 

机构地区：[1]吉林大学第一医院临床流行病学研究中心,吉林长春130021 [2]吉林大学第一医院病理诊断中心,吉林长春130021 [3]吉林大学第一医院肝胆胰内科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2014年第2期351-357,I0004,共8页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81001312;81072347;81202377);吉林省科技厅科研项目资助课题(201215035)

摘　　要：目的:探讨原发性肝细胞癌(HCC)患者肿瘤组织及其瘤旁组织中半乳糖凝集素3(Galectin-3)和半乳糖凝集素9(Galectin-9)的表达及其与预后的关系,阐明Galectin-3和Galectin-9在HCC发生发展过程中的意义。方法:采用免疫组织化学法检测197例HCC患者肿瘤组织和131例瘤旁肝组织中Galectin-3和Galectin-9的表达水平;分析2种蛋白的表达与患者临床病理特征和预后的关系。结果:Galectin-9表达阳性率在HCC肿瘤组织和瘤旁组织中差异无统计学意义(P>0.05),高分化肿瘤组织中Galectin-9表达水平明显高于低分化肿瘤组织(P<0.05),脉管浸润者Galectin-9表达水平低于无脉管浸润者(P<0.05);Galectin-9高表达患者术后生存时间显著长于低表达和阴性表达患者(P<0.05)。肿瘤组织中Galectin-3表达阳性率显著高于瘤旁组织(P<0.01),但其表达水平与患者各临床病理特征及术后长期生存无关联。调整影响因素后多因素分析,仅丙肝感染(RR=2.86,95%CI:1.13~7.30,P=0.027)、多发肿瘤(RR=2.38,95%CI:1.39~4.07,P=0.002)、肿瘤直径>5cm(RR=2.13,95%CI:1.21~3.76,P=0.009)和脉管浸润(RR=1.96,95%CI:1.07~3.62,P=0.030)成为影响HCC患者术后长期生存的独立影响因素。结论:Galectin-9表达水平与HCC患者肿瘤组织分化程度和脉管浸润有关联,但Galectin-9和Galectin-3表达水平与HCC患者的预后无关联。Objective To investigate the expressions of Galectin-3 and Galectin-9 in the tumor tissue and adjacent cancer tissue of the patients with hepatocellular carcinoma （HCC） and their relationship with prognosis, and to explore the significance of Galectin-3 and Galectin-9 in the occurrence and development of HCC. Methods The expressions of Galectin-3 and Galectin-9 in 197 cases of HCC tissue and 131 cases of adjacent cancer tissue were detected by immunohistochemical technique; the association between the expressions of Galectin-3 and Galectin-9 and the clinicopathologic characteristics and prognosis of HCC patients were analyzed. Results There was no significant difference of the positive expression rate of Galectin-9 between HCC tumor tissue and adjacent cancer tissue （P^0.05）. The expression levels of Galectin-9 in the patients with poorly differentiated HCC or with lymph- vascular invasion were lower than those in the patients with well differentiated HCC or without lymph-vascular invasion （P~ 0.05）3 the patients with a high-level expression of Galection-9 showed a longer survival period compared with those with negative and low-level expression of Galectin-9 （P〈 0.05）. The positive expression rate of Galectin-3 in HCC tumor tissue was significant higher than that in adjacent cancer tissue （P〈 0.01）. The expression level of Galectin-3 in HCC tissue was not related to the clinicopathologic characteristics and prognosis of the patients. After adjusting the influencing factors, the multivariate analysis results showed only the infection of hepatitisc （RR=2.86, 95%CI： 1.13-7.30, P= 0. 027）, multiple tumor （RR= 2.38, 95%CI： 1.39-4.07, P=0. 002）, diameter of tumor〉 5 cm （RR = 2.13, 95% CI： 1.21 - 3.76, P = 0. 009）, and lymph-vascular invasion（RR= 1.96, 95%CI： 1.07-3.62, P = 0. 030） were the independent influencing factors of prognosis of the HCC patients. Oonclusion The expression level of Galectin-9 is related to the tumor differentiation and lymph- vascular invasion o

关 键 词：肝细胞肿瘤 半乳糖凝集素3 半乳糖凝集素9 预后 

分 类 号：R575[医药卫生—消化系统]