心律宁胶囊中齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷浓度HPLC检测方法的建立及评价  

Establishment and evaluation of HPLC method for detection of concentration of oleanolic acid-3-O-β-D- pyran glucuronide in Xinlüning capsule

作　　者：王淑琴[1] 崔东滨[1] 

机构地区：[1]吉林省中医药科学院,吉林长春130012

出　　处：《吉林大学学报（医学版）》2014年第2期446-449,共4页Journal of Jilin University：Medicine Edition

基　　金：国家科技重大专项资助课题(2011ZX09401-305-47)

摘　　要：目的：采用高效液相色谱（HPLC）法测定心律宁胶囊中齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷的浓度，探讨影响心律宁胶囊中齐墩果酸-3-O-β-D-喃葡萄糖醛酸苷浓度测定的各种因素。方法：采用HPLC法测定齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷浓度。色谱柱为HypersilC18柱（4．6mm×250．0mm，5．0μm），流动相为乙腈-1％冰醋酸（56：44），检测波长为210nm，速流为I．00mL·min^-1，柱温30℃。结果：在本色谱条件下，齐墩果酸-3-O-β-D-喃葡萄糖醛酸苷的分离效果良好，在14．80～26．64μg内呈良好的线性关系（r=0．9999），平均加样回收率为97．4％，相对标准偏差（RSD）为1．4％。结论：采用HPLC法测定心律宁胶囊中齐墩果酸-3-O-β-D-喃葡萄糖醛酸苷浓度简便、准确、专属性强，HPLC可作为心律宁胶囊浓度测定的质量控制方法。Objective To establish an high performance liquid chromatography （HPLC） method for the determination of oleanolic acid-3-O-β-D- pyran glucuronide concentration in Xinliining capsule, and to examine the various factors that affect the determination of oleanolic acid-3-O-β-D-pyran glucuronide concentration in Xinltining capsule. Methods HPLC method was used to detect the concentrantion of oleanolic acid-3-O-β-D-pyran glucuronide. Chromatogyaphic assay was performed with HypersilC18 （4.6 mm× 250.0 mm, 5.0 μm）column and acetonitrile-1G glacial acetic acid （56 ： 44）. The column temperature was set at 30℃ and the wavelength was 210 nm and the flow rate was 1.00 mL ·min^-1. Results Under this condition, the recorery rate of oleanolic acid-3-O-β-D- pyran glucuronide was 97.4% with the relative standard deviation（RSD） of 1.4%. The calibration curve showed good linearity in the range of 14.80-26.64μg（γ=0. 999 9）. Conclusion The detection of the concentration of oleanolic acid-3-O-β-D-pyran glucuronide in Xinltining capsule by HPLC method is simple and accurate, which can be used as the quality control method of Xingltining capsule.

关 键 词：心律宁胶囊 齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷 色谱法 高效液相 

分 类 号：R914[医药卫生—药物化学]