参命源皂甙人参锭中人参皂苷浓度HPLC检测方法的建立及评价  

Establishment and evaluation of HPLC method for determination of concentrations of ginsenosides in Life-Source Ginsenosides Complex

作　　者：刘竞研[1] 尹建元[1] 张大伟 杨玉霞[1] 陈凡波 孙丹丹[1] 孙振学[3] 赵芸浩 孟勤[4] 

机构地区：[1]吉林大学药学院天然药物化学教研室,吉林长春130021 [2]明参生物科技股份有限公司,中国台湾竹北30263 [3]吉林油田总医院药学部,吉林松原138000 [4]吉林大学药学院药学实验中心,吉林长春130021 [5]东北师范大学附属中学

出　　处：《吉林大学学报（医学版）》2014年第2期450-453,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划医药产业发展专项资金资助课题(YYZX201123-4)

摘　　要：目的：建立测定参命源皂甙人参锭中5种人参皂苷（Rg1、Re、Rb1、Rbz和Rd）浓度的高效液相色谱（HPLC）法，为工业生产的质量控制提供依据。方法：采用HPLC法测定参命源皂甙人参锭中人参皂苷的浓度，检测条件：AlltimaC18（250．0mm×4．6mm，5．0μm）色谱柱，以乙腈为流动相A，0．1％磷酸水溶液为流动相B，梯度洗脱；流速为1．0mL·min^-1；检测波长为203nm；柱温为40℃。结果：人参皂苷Rg1、Re、Rb1、Rb2和Rd的线性关系良好，相关系数（r）均大于0．9991，平均回收率为99．5％～102．6％；精密度实验、稳定性实验和重复性实验检测人参皂苷Rg1、Re、Rb1、Rb2和Rd的相对标准差（RSD）分别为0．35％～0．76％、0．74％～1．44％和0．78％～1．47％。结论：HPLC法检测参命源皂甙人参锭中人参皂苷浓度的方法准确、简单可行、重复性好，可以用于参命源皂甙人参锭的质量控制。Objective To establish an high performance liquid chromatography （HPLC） method for the determination of concentrations of five active ginsenosides （Rg1, Re, Rb1, Rb2, and Rd）in Life-Source Ginsenosides Complex, and to provide basis for the quality control of industrial production. Methods HPLC method was used to determine the concentrations of five ginsenosides in Life-Source Ginsenosides Complex. The developed conditions of HPLC were as follows： Alltima C18 （250.0 mm × 4.6 mm, 5.0μm） column; A gradient elution was applied by mixing mobile phase A and B, which consisted of acetonitrile and 0.1% phosphoric acid, respectively; the flow rate was 1.0 mL· min^-1, and the detector wave length was 203 nm and the column temperature was 40℃. Results The calibration curve showed good linearity, and the values of correlation coefficient were higher than 0. 999 1 for all the analytes, and the average recovery rates were ranged from 99.5% to 102.6%. The relative standard deviation （RSD） detected by accuracy, stability and repetability tests of five ginsenosides were 0.35 M - 0.76 %, 0.74 %-1.44 %, and 0.78 % - 1.47%, respectively. Oonclusion The detection of ginsenosides in Life-Soure Ginsenosides Complex with HPLC method is accurate, easy and reproducible, which can be applied to quality control of the Life-Source Ginsenosides Complex.

关 键 词：参命源皂甙人参锭 人参皂苷 色谱法 高效液相 

分 类 号：R914[医药卫生—药物化学] R194[医药卫生—药学]