壳聚糖-有机累托石/海藻酸钠复合纳米粒的制备  

Preparation of Quaternized Chitosan (QC)/alginate (ALG) Complex Nanoparticles

作　　者：白录军 周天 冯旭阳[2] 郑鹏飞[2] 徐瑞芬[3] 

机构地区：[1]兰州军区临潼疗养院第二疗养区,陕西临潼710600 [2]第四军医大学西京医院心脏内科,陕西西安710032 [3]西安交通大学生命科学院技术学院,陕西西安710049

出　　处：《现代生物医学进展》2014年第11期2029-2031,2036,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(8100597;81070248)

摘　　要：目的:以BSA作为模型药物,制备壳聚糖季铵盐-OREC复合物纳米微粒,建立一种安全有效的药物控释传递系统。方法:超声条件下,制备不同质量比的具有壳聚糖硅酸盐插层结构的复合物纳米微粒,观察其形态学特征、进行红外光谱分析。同时,测定OREC对BSA包封率和载药量的影响。结果:成功制备了不同质量比的OREC-HTCC纳米粒子。电镜结果显示纳米粒呈圆球形,均匀,平均粒径约为30nm。红外图谱分析证实,HTCC插入了OREC插层中,BSA成功地包裹入HTCC-ALG/OREC混合材料制备的纳米微粒。加入OREC后,纳米粒子的包封率及载药量均明显提高,但随着加入量的增加,包封率及载药量逐渐减少。结论:OREC-HTCC纳米粒子是良好的蛋白药物载体,具有粒径小、包封率高、缓释效果好等优点,为CS-OREC作为潜在的药物给药系统的进一步应用提供科学依据。Objective： To preparation of quatemized chitosan （QC）/alginate （ALG） complex nanoparticles with BSA. Therefore, it is necessary to establish an approach for fabricating an efficient controlled release drug delivery system. Methods： Under the condition of ultrasonic, preparation of different mass ratio with silicate chitosan intercalated structure complex nanoparticles. Morphology, infrared spectrum analysis, and the encapsulation efficiency and the loading capacity of nanoparticles were investigated. Results： OREC-HTCC nanoparticles were prepared successfully and universally spherical with a diameter of approximately 30 nm. FT-IR results confirmed that HTCC chains had intercalated into the interlayer of OREC and BSA was encapsulated by nanoparticles prepared by HTCC-ALG/OREC mixed material. After introduction of OREC, encapsulation efficiency and drug load of nanoparticles were significantly enhanced. How-ever, encapsulation efficiency and drug load declined with the increase of OREC introduction. Conclusion： The nanoparticles prepared by HTCC-OREC have been used as drug carrier for protein, have the advantages of small size, high encapsulation efficiency and slow re-lease. It provides scientific data for modulating the release of oral drug. It could be expected to be potential drug delivery system though there were few related reports.

关 键 词：壳聚糖 纳米粒 有机累托石 

分 类 号：Q819[生物学—生物工程] R914.2[医药卫生—药物化学]