FAPα在胞外信号传递中与整合素的关系  

The Relationship between FAP α in Extracellular Signal Transduction and Integri

作　　者：田甜[1] 温秋婷[2] 杨薇薇[1] 李春红[1] 郑芳[1] 蒋淑婉[1] 李云[1] 刘端阳[1] 曲雪梅[1] 陈鹤[1] 

机构地区：[1]哈尔滨医科大学病理教研室,黑龙江哈尔滨150081 [2]齐齐哈尔医学院病理诊断中心,黑龙江齐齐哈尔161006

出　　处：《现代生物医学进展》2014年第11期2058-2061,共4页Progress in Modern Biomedicine

基　　金：黑龙江省自然科学基金(D201049)

摘　　要：目的:研究成纤维激活蛋白(Fibroblast Activation Protein,FAP)在促进卵巢癌细胞发生侵袭、迁移、增殖过程中与整合素α3β1、尿激酶型纤溶酶原激活剂受体(uPAR)的关系。方法:1).免疫共沉淀共同检测整合素α3β1、uPAR在HO-8910PM上是否为二聚体。2).transwell侵袭实验、迁移实验检测抑制整合素α3β1、uPAR后,卵巢癌细胞系HO-8910PM的侵袭迁移能力;3).抑制整合素α3β1、uPAR后,再给予FAPα对HO-8910PM侵袭、迁移和增殖的影响。结果:1).整合素α3β1、uPAR在HO-8910PM细胞外同一个位置表达;2).抑制整合素α3β1能够明显抑制HO-8910PM的侵袭、迁移、增殖能力,并且可以抑制FAP对肿瘤的作用。3).PAI-1抑制uPAR后,HO-8910PM的侵袭、迁移、增殖无明显变化,同时对FAP也无明显作用。结论:整合素α3β1和uPAR在HO-8910PM是一个复合体,FAPα在细胞外是通过整合素α3β1传递信号进入细胞内而不是通过uPAR,整合素α3β1是通过uPAR与肿瘤细胞相连接。Objective： To study the relationship of integrin α3β1, urokinase-type plasminogen activator receptor （uPAR） and fibroblast activation protein （Fibroblast Activation Protein, FAP） in promoting invasion, migration, proliferation of ovarian cancer cells. Methods： 1） Co-immunoprecipitation was used to detected to whether integrin α3β1 uPAR was a trimer of HO-8910PM; 2） Transwell invasion assay, migration assay and MTT assay were to detect the impact on the invasion, migration and proliferation of HO-8910PM, which imposed by inhibition of integrin α3β1, uPAR alone and with combined effects of FAP; 3 ） Inhibition of integrin α3β1, uPAR, and then give FAPα on HO-8910PM invasion, migration and proliferation. Results： 1 ）Integrin α3β1, uPAR in HO-8910PM expression of extracellular same position; 2） Inhibiting integrin α3β1 can inhibit HO-8910PM invasion, migration, proliferation, and can inhibit the FAPα function; 3） PAI-1 inhibition of uPAR, the ability of HO-8910PM invasion, migration, proliferation reduced and didn＇t have any significant changes on the FAPα＇ function. Conclusions： Integrinα3β1 and uPAR in HO-8910PM is a complex, FAPα in the extracellular pass through integrin α3β1 signal into the cell rather than through uPAR, integrins α3β1 by uPAR connected with tumor cells.

关 键 词：成纤维细胞激活蛋白(FAP) 卵巢癌细胞系(HO-8910PM) 整合素Α3Β1 尿激酶型纤溶酶原激活剂受体(uPAR) 

分 类 号：R730.231[医药卫生—肿瘤]