结直肠癌组织中血管内皮生长因子-C及nm23-H1蛋白的表达及意义  

The expression and clinical significance of vascular endothelial growth factor-C and nm23-H1 in colorectal carcinoma

作　　者：焦彦华[1] 王秀艳[1] 刘叶廷 

机构地区：[1]河北省邢台市第一医院邢台医学高等专科学校第一附属医院检验科,054002

出　　处：《中国医师进修杂志》2014年第8期20-23,共4页Chinese Journal of Postgraduates of Medicine

摘　　要：目的 探讨结直肠癌组织中血管内皮生长因子（VEGF）-C和nm23-H1蛋白的表达水平及其临床意义.方法 选取120例结直肠癌患者肿瘤组织,并以55例癌旁正常组织作为对照;采用免疫组织化学法检测组织中VEGF-C和nm23-H1蛋白的表达水平,分析VEGF-C和nm23-H1蛋白的表达与结直肠癌患者临床特征及预后的关系.结果 VEGF-C在结直肠癌组织中的阳性表达率为71.7％（86/120）,高于癌旁正常组织的21.8％（12/55）,差异有统计学意义（Х^2=35.186,P＜0.01）.nm23-H1蛋白在结直肠癌组织中的阳性表达率为57.5％ （69/120）,低于癌旁正常组织的90.9％ （50/55）,差异有统计学意义（Х^2=18.351,P＜0.01）.VEGF-C阳性表达仅与结直肠癌患者的淋巴结转移有相关性（P＜0.05）,nm23-H1蛋白阳性表达与结直肠癌患者的淋巴结转移和病理学类型有相关性（P＜ 0.01或＜0.05）,VEGF-C和nm23-H1蛋白的阳性表达与性别、年龄、肿瘤直径、原发部位和浸润深度均无相关性（P＞0.05）.Spearman相关分析结果显示,在结直肠癌组织中,VEGF-C与nm23-H1蛋白的阳性表达呈负相关（r=-0.472,P＜0.01）.结论 VEGF-C和nm23-H1蛋白联合检测对预测结直肠癌患者的预后具有重要的临床意义,但是否可作为判断结直肠癌患者预后的标志物仍需进一步研究.Objective To discuss the expression and clinical significance of vascular endothelial growth factor （VEGF）-C and nm23-H1 in colorectal carcinoma.Methods Immunohistochemical staining was employed to determine the expression of VEGF-C and nm23-H1 in the carcinoma tissues of 120 cases with colorectal carcinoma and in the adjacent mucosal tissues of 55 cases as control,and analyzed their correlation with cliniopathological features and prognosis.Results The positive expression of VEGF-C in the carcinoma tissues was 71.7% （86/120）,significantly higher than that in the adjacent mucosal tissues [21.8 % （12/55）],and there was significant difference （Х^2 =35.186,P 〈 0.01）.The positive expression of nm23-H1 in the carcinoma tissues was 57.5% （69/120）,significantly lower than that in the adjacent mucosal tissues [90.9% （50/55）],and there was significant difference （Х^2 =18.351,P 〈 0.01）.The expression of VEGF-C was significantly correlated with lymph node metastasis （P 〈 0.05）,and the expression of nm23-H 1was significantly correlated with lymph node metastasis and pathological type （P 〈0.01 or 〈0.05）.The expression of VEGF-C and nm23-H1 did not show a significant correlation with gender,age,tumor size,tumor site and depth of invasion （P 〉 0.05）.Spearman correlation analysis showed that the expression of VEGF-C was negatively correlated with the expression of nm23-H 1 in colorectal carcinoma （r =-0.472,P 〈0.01）.Conclusions The joint detection of VEGF-C and nm23-H1 expression is very promising in prediction of the prognosis of patients with colorectal carcinoma.However,whether it can be used as a marker in prognosis judgment need further investigation.

关 键 词：结直肠肿瘤 肿瘤分期 预后 血管内皮生长因子C NM23-H1蛋白 

分 类 号：R735.34[医药卫生—肿瘤]