鸡OVR基因5′侧翼区多态性与鸡蛋胆固醇含量的关联分析  

Association between polymorphism in the 5′ flank region of oocyte vitellogensis receptor gene and egg cholesterol content

作　　者：朱世康[1] 吕世杰[1] 刘向波[1] 田亚东[1,2] 蒋瑞瑞[1,2] 康相涛[1,2] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450000 [2]河南省家禽种质资源创新工程研究中心,河南郑州450000

出　　处：《西北农林科技大学学报（自然科学版）》2014年第3期10-16,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：河南省科技创新人才(杰出青年)基金项目(104100510013);河南省博士后科研项目(2011034)

摘　　要：【目的】分析鸡卵母细胞卵黄生成受体(OVR)基因5′侧翼区多态性与鸡蛋胆固醇含量的关联性。【方法】以丝羽乌骨鸡为研究对象,测定936枚丝羽乌骨鸡蛋黄和全蛋胆固醇含量,利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法结合PCR产物测序技术,对丝羽乌骨鸡OVR基因5′侧翼区进行多态性检测,并分析其多态性与鸡蛋胆固醇含量的关联性。【结果】丝羽乌骨鸡蛋黄胆固醇含量为13.411mg/g,全蛋胆固醇含量平均为456.502mg/hg。在鸡OVR基因5′侧翼区检测到g.9511G>A和g.10428T>C 2个突变位点,其与鸡蛋蛋黄和全蛋胆固醇含量显著或极显著相关,胆固醇含量较低的基因型分别是GG和TT(P<0.05)。【结论】鸡OVR基因5′侧翼区多态性对鸡蛋胆固醇含量有一定影响。[Objective] This study aimed to analyze the association between the polymorphism in 5p flank region of chicken oocyte vitellogensis receptor gene （OVR） and egg cholesterol content. [Method] Silky fowls were used as the research subjects. A total of 936 silky eggs were used to measure the choles- terol contents in yolk and the whole egg. Polymerase chain reaction-restriction fragment length polymor- phism （PCR-RFLP） and PCR products sequencing method were used to detect the polymorphisms （SNPs） in the 5~ flank region of the OVR gene,and analyze the association between SNPs and egg cholesterol con- tent. [Result] The average cholesterol contents in yolk and the whole egg were 13. 411 mg/g and 456. 502 mg/hg,respectively. Two SNPs （g. 9511G^A and g. 10428T）C） were significantly or extremely signifi- cantly associated with the cholesterol contents in yolk and the whole egg. Genotypes GG and TT had the lowest cholesterol contents （P^0.05）. [Conclusion] OVR gene had certain effects on the egg cholesterol content.

关 键 词：丝羽乌骨鸡 OVR基因5′侧翼区 SNPS 鸡蛋胆固醇含量 

分 类 号：S831.2[农业科学—畜牧学]