Delisheng Injection(得力生注射液),A Chinese Medicinal Compound, Enhanced the Effect of Cis-platinum on Lung Carcinoma Cell Line PGCL3  

作　　者：董西林 宫英 陈真真 王雅娟 

机构地区：[1]Department of Respiratory,the Second Affiliated Hospital,College of Medicine of Xi'an Jiaotong University [2]Department of Pharmacy,the Fourth People's Hospital of Ji'nan City [3]Department of Respiratory,Zibo Central Hospital

出　　处：《Chinese Journal of Integrative Medicine》2014年第4期286-291,共6页中国结合医学杂志（英文版）

基　　金：Supported by the Project of Science and Technology Development in Shaanxi Province of China(No.2008KG10-01)

摘　　要：Objective:To investigate the effect of Delisheng Injection（得力生注射液 DLS）,a Chinese medicinal compound,DLS combined with cis-platinum（DDP）,an active agent used in lung cancer chemotherapy,on a human highly metastatic giant lung carcinoma cell line PGCL3.Methods:The suspended PGCL3 cells at10;/mL cultured in 96-well tissue culture plates were divided into 4 groups:DLS treatment group（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL）,DDP treatment group（1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL）,combined DLS with DDP treatment group（DLS:DDP 2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL）and a control group.The cytotoxicity of DLS with different concentrations（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL）on PGCL3 cells was determined by 3-（4,5）-dimethylthiahiazo（-z-y1）-3,5-di-phenytetrazoliumromide（MTT） assay.Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay.Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells.Results:Compared with the control group,DLS（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL） could significantly decrease cell proliferation,adhesion,invasion and migration abilities（P<0.05）.Cell adhesion,invasion and migration abilities were significantly decreased after combination treatment of DLS:DDP（2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL） compared with DDP single-agent treatment（1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL,P<0.05）,respectively.Conclusions:DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis.Our research provided a experimental basis about the treatment on highly metastatic lung caner.Objective： To investigate the effect of Delisheng Injection （得力生注射液, DLS）, a Chinesemedicinal compound, DLS combined with cis-platinum （DDP）, an active agent used in lung cancer chemotherapy,on a human highly metastatic giant lung carcinoma cell line PGCL3. Methods： The suspended PGCL3 cells at105/mL cultured in 96-well tissue culture plates were divided into 4 groups： DLS treatment group （2 tμ I_/mL, 5 μ L/mL,10 μ/mL, 25 p.UmL）, DDP treatment group （1 μg/mL, 2 p.g/mL, 5 lμg/mL, 15 μg/mL）, combined DLS with DDPtreatment group （DLS：DDP 2 μ L/mL：I iμ g/mL, 5 μ L/mL：2 μL/mL, 10 p. L/mL：5 μg/mL, 25 iμ L./mL：15 μ g/mL）anda control group. The cytotoxicity of DLS with different concentrations （2 μ L/mL, 5 μ L/mL, 10 μ L/mL, 25 μ L/mL）on PGCL3 cells was determined by 3-（4,5）-dimethylthiahiazo（-z-y1）-3,5-di-phenytetrazoliumromide （MTT） assay.Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay. Chemotactic movementmodel of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells.Results： Compared with the control group, DLS （2 μ L/mL, 5 μ L/mL, 10 μL/mL, 25 μ L/mL） could significantlydecrease cell proliferation, adhesion, invasion and migration abilities （P〈0.05）. Cell adhesion, invasion andmigration abilities were significantly decreased after combination treatment of DLS：DDP （2 p. L/mL 5 μ g/mL,5 μ L/mL：2 μg/mL, 10 μ L/mL：5 μg/mL, 25 μL/mL：15 μg/mL）compared with DDP single-agent treatment（1 μ g/mL, 2 iμ g/mL, 5 p. g/mL, 15 p. g/mL, P〈0.05）, respectively. Conclusions： DLS single-agent has a satisfyinginhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis.Our research provided a experimental basis about the treatment on highly metastatic lung caner.

关 键 词：lung cancer delisheng CIS-PLATINUM PGCL3 

分 类 号：R734.2[医药卫生—肿瘤]