2-硫代巴比妥酸修饰金纳米探针高灵敏比色检测三聚氰胺  

Using 2-Thiobarbituric Acid-modified Gold Nanoparticles as Colorimetric Probes for Sensitive Detection of Melamine

作　　者：费瑶[1] 郭涵[2] 奚焕祥 陈婉熠 蔡琳熠 王爱军[1] 冯九菊[1,2] 

机构地区：[1]浙江师范大学化学与生命科学学院,金华321004 [2]河南师范大学化学化工学院,新乡453007

出　　处：《应用化学》2014年第4期496-501,共6页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金资助项目(21175118;21275130)~~

摘　　要：以2.硫代巴比妥酸（TBA）修饰金纳米粒子为探针，TBA与三聚氰胺通过氢键作用诱导金纳米探针团聚，进而使金纳米胶体颜色由酒红色变为蓝色。实验优化得最佳反应条件为在乙酸缓冲溶液（pH=7.0）介质中，室温反应15min。对不同浓度三聚氰胺进行检测时发现，在0.062～0.18 μmol／L和0.18～6.0 μmol／L之间，A。／A，如吸收比率与三聚氰胺浓度呈现良好的线性关系，检出限为0.043 μmol／L。该方法用于检测牛奶样品中的三聚氰胺的加标回收率为102.8％～105.3％。A simple and rapid colorimetric method was developed for the detection of melamine using 2-thiobarbituric acid-modified gold nanoparticles （TBA-AuNPs） as probes. Melamine can induce the aggregation of TBA-AuNPs via the hydrogen-bonding interactions in acetic acid buffer solutions （ pH = 7 ） , resulting in the color change from wine-red to blue within 15 min at room temperature. Under optimized conditions, the absorption ratio of the A660/A520 shows good linear relationship with the melamine concentration in the range of 0.062 ～ 0. 18 μmol/L and 0.18 ～ 6.0 μmol/L, respectively, with the detection limit of 0. 043 μmol/L. The recoveries of the method are within the range of 102.8% ～ 105.3%. It provides a platform for rapid, selective and sensitive colorimetric detection of melamine in milk samples.

关 键 词：三聚氰胺 硫代巴比妥酸 金纳米探针 比色法 

分 类 号：O657.3[理学—分析化学]