miR-760靶向CDK8基因对人乳腺癌细胞MDA-MB-231增殖与迁移的影响  

miR-760 inhibits proliferation and migration by targeting CDK8 in human breast cancer cell line MDA-MB-231

作　　者：李晓宇[1] 罗颀枫[1] 房林[1] 

机构地区：[1]同济大学附属第十人民医院甲状腺乳腺外科,上海200072

出　　处：《同济大学学报（医学版）》2014年第1期1-6,共6页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金(81272240)

摘　　要：目的探讨miR-760对人乳腺癌细胞中CDK8蛋白表达影响及其在乳腺癌细胞MDA-MB-231中的功能。方法使用实时定量PCR方法检测miR-760在人乳腺癌细胞中的表达;使用脂质体介导的miRNA转染、Western印迹法、MTT法、流式细胞仪技术分别检测转染miR-760前后CDK8蛋白表达差异及其对乳腺癌细胞增殖、迁移等细胞功能和细胞周期的影响。结果人乳腺癌细胞中miR-760表达水平较正常乳腺细胞降低;外源性上调miR-760可有效抑制人乳腺癌细胞株MDA-MB-231 CDK8蛋白表达(抑制效率为46.3%,P<0.05);人乳腺癌细胞株MDA-MB-231转染miR-760后,其细胞增殖、迁移能力显著降低(P<0.05)。结论 miR-760可能通过靶向CDK8影响人乳腺癌细胞增殖与迁移,在乳腺肿瘤发生发展中起到抑癌基因作用。Objective To investigate the effects of miR-760 on CDK8 protein expression, cell proliferation and migration in human breast cancer MDA-MB-231 cell line. Methods The expression of miR-760 was quantitatively examined by Real-Time PCR in human breast cancer MDA-MB-231 cells and normal breast cells, miR-760 was transfected in MDA-MB-231 cells with lipofection; the expression level of CDK8 protein, cell proliferation, cell cycle, cell migration of transfected MDA- MB-231 cells were assayed by Western blotting, MTT, flow cytometry and Transwell test, respectively. Results The expression level of miR-760 was significantly decreased in the breast cancer cells compared with normal breast cells. After transfection of miR-760 mimics, the capacities of breast cancer cell proliferation and migration were significantly reduced; and the expression of CDK8 protein was inhibited （P 〈 0.05 ）. Conclusion miR-760 can inhibit the cell proliferation and migration ofhuman breast cancer MDA-MB-231 cells by targeting CDK8.

关 键 词：乳腺肿瘤 miR-760 CDK8基因 

分 类 号：R363[医药卫生—病理学]