嘌呤受体P2X_7对视网膜毛细血管周细胞凋亡的影响  

Effect of purine receptor P2X_7 in pericyte of retinal microvascular apoptosis

作　　者：左炜[1] 祝芸芸[1] 于洪涛 利焕廉 周金文[1] 王恒[1] 

机构地区：[1]中国广东省深圳市第四人民医院眼科,518033

出　　处：《国际眼科杂志》2014年第4期603-605,共3页International Eye Science

基　　金：深圳市科技项目(No.201103172)~~

摘　　要：目的:探讨嘌呤受体P2X7在体外培养大鼠视网膜毛细血管周细胞上的功能意义。方法:利用显微镜下观察结合TUNEL计数法分别测定P2X7激动剂BzATP和拮抗剂OxATP对体外培养的正常视网膜毛细血管周细胞以及糖尿病大鼠视网膜毛细血管周细胞存活率的影响。结果:P2X7受体激动后可介导视网膜毛细血管周细胞死亡,糖尿病大鼠视网膜毛细血管周细胞较正常视网膜毛细血管周细胞,在同等激动剂浓度下或在同样的时间点,能引起更多的细胞凋亡。而拮抗剂OxATP可以明显减轻BzATP对视网膜毛细血管周细胞的毒性作用,提高其存活率。结论:P2X7受体活性对视网膜毛细血管周细胞凋亡有一定调控作用。AIM： To discuss the functional significance of purine receptor P2X7 in retinal capillary pericytes rats. METHODS：The TUNEL test was applied to observe the survival rate of normal retinal capillary pericytes and diabetic retinal capillary pericytes which were affected by P2X7 agonist BzATP and antagonist OxATP in microscopy. RESULTS： Activation of P2X7 receptors caused occurence of apoptosis in retinal capillary pericytes. Compared with normal retinal capillary pericytes, when they were at the same concentration or time, the diabetic retinal capillary pericytes caused much more apoptosis than normal retinal capillary pericytes. The antagonist OxATP could significantly reduce the toxic effects activated by BzATP, and then increased the livability of retinal capillary pericytes. CONCLUSION：The activity of P2X7 receptor plays a regulatory role on RPC apoptosis.

关 键 词：P2X7受体 视网膜微血管周细胞 糖尿病视网膜病变 凋亡 

分 类 号：R774.1[医药卫生—眼科]