Snail,E-cadherin在结直肠癌中的表达及意义  

Expression and Significance of Snail and E-cadherin in Colorectal Cancer

作　　者：孙华聪 阎宏[2] 李登欣[3] 刘丽荣[1] 

机构地区：[1]潍坊医学院内科学教研室,山东潍坊261053 [2]山东经贸职业学院 [3]潍坊市人民医院消化内科

出　　处：《潍坊医学院学报》2014年第1期51-53,共3页Acta Academiae Medicinae Weifang

摘　　要：目的观察锌指转录因子（Snail）和土皮性钙粘附蛋白E-cadherin在结直肠癌组织中的表速情况，并探讨两者关系及意义。方法采用免疫组织化学PV9000法检测50例结直肠癌患者手术切除的肿瘤组织及20倒癌旁正常结直肠组织中Snail，E-cadherin蛋白的表达。结果结直肠癌纽织中Snail的阳性表达率明显高于癌旁正常结直肠组织（P〈0．05），而结直肠癌组织中E-cadherin的阳性表达率显著低于癌旁正常结直肠组织（P〈0．05）；Snail和E-cad在结直肠癌组织中的表达均与癌组织的分化程度、浸润深度、临床分期、是否有淋巴结转移及远处器官转移密切相关（P〈0．05）；在结直肠癌组织中Snail与E-cadherin的表达呈负相关（P〈0．05）。结论Snail和E-cadherin的异常表达与结直肠癌的发生、发展及侵袭转移密切相关，联合检测两种蛋白对于结直肠癌的早期诊断及预后判断有重要意义。Objective To study the expression of Snail and E-cad protein in coloreeta] cancer,and to explore the relationship and significance between them. Methods The expression of Snail and E-cadherin protein was detected by using immunohistochemica] PV9000 metheod in cases of surgical removal of the colorectal cancer tissue and cases of para-cancerous normal gastric tissue. Results The positive rate of Snail immunostaining in colorectal cancer was higher than that in adjacent normal colorectal tissue（ P 〈 0.05 ） ,and the posi- tive rate of E-cadherin immunostaining in colorecta] cancer was lower than that in adjacent normal colorectal tissue （ P 〈 0.05 ）. The expres- sion of Snail and E-cadherin in colorectal cancer tissue was closely related with the depth of tumor invasion, differentiatial degree, clinical stage,lymph node metastases and distant metastases （P 〈 O. 05 ）. There was negative correction between the expression of Snail and E-cadher- in protein in colorectal cancer（ P 〈 0.05 ）. Conclusion The abnormal expressions of Snail and E-cadherin are closely related to the occur- rence, development, invasion and metastasis of colorectal cancer. Co-detection of Snail and E-eadherin has significant effect on early diagnosis and prognosis of colorectal cancer.

关 键 词：结直肠肿瘤 SNAIL蛋白 E—cadherin蛋白 免疫组织化学技术 

分 类 号：R735.3[医药卫生—肿瘤]