环氧合酶-2基因-1195G>A多态性位点与缺血性脑卒中患者阿司匹林抵抗的相关性  

Correlation between Single Nucleotide Polymorphism of-1195G >A of COX-2 Gene and Aspirin Resistance in Patients with Ischemic Stroke

作　　者：陈忠云[1] 李珊珊[1] 李婧[1] 徐志伟[1] 杨旭[1] 

机构地区：[1]航天中心医院(北京大学航天临床医学院)神经内科,北京100049

出　　处：《神经损伤与功能重建》2014年第2期111-114,共4页Neural Injury and Functional Reconstruction

摘　　要：目的：探讨环氧合酶-2（COX-2）基因-1195 G＜A多态性位点与缺血性脑卒中（IS）患者阿司匹林抵抗（AR）的相关性。方法：纳入中国北方汉族IS患者210例，根据血小板聚集率分为阿司匹林敏感（AS）组147例，非AS组63例。采用扩增阻碍突变系统（ARMS）结合TaqMan探针的实时荧光定量PCR方法检测-1195 G＜A多态性。结果：-1195G＜A位点基因型、显性模型分布频率在AS组及非AS组中差异均无统计学意义（P＞0.05）；非AS组中-1195G＜A位点（GA＋AA）基因型频率及A等位基因频率高于AS组，差异均有统计学意义（x^2=4.96，=0.03；x^2=5.50，P=0.02）；多因素分析提示，与GG基因型相比，COX-2的-1195G＜A位点（GA＋AA）基因型发生AR可能性更大（OR=2．41，95％CI为1．09—5．37，P=0．03）。结论：COX-2基因—1195G〉A位点遗传变异与可能Is患者发生AR相关，可能是AR发生的一个遗传易感性位点。ObjectiveTo study the correlation between single nucleotide polymorphisms （SNPs） of-1195G〈A of cyclooxygenase-2 （COX-2） gene and Aspirin resistance （AR） in patients with ischemic stroke （IS）. Methods：According to the rate of platelet aggregation, 210 northern Chinese Han patients with IS were divided into Aspirin sensitivity （AS） group （147 cases） and non-Asgroup [17 AR cases, and 46 Aspirin semi-resistance （ASR） cases] （n=63）. The SNPs were detected by Amplification mutation system （ARMS） combined with TaqMan probe in a real-time PCR. Results：There were no significant differences in the genotype, allele, additive or recessive model frequencies of-1195G〈A polymorphisms between the 2 groups. The significant difference between （GA＋AA） and GG genotype frequencies of-1195G〈A polymorphisms were observed in groups of AS and non-AS （P =0.03）. For A allele of-1195G〈A, there was also a significant difference between the two groups （P =0.02）. On multivariate analysis controlling for conventional vascular risk factors, the-1195 GA and AA genotypes were identified inde-pendently to be related with （ASR＋AR） （ OR=2.41,：1.07-5.42, =0.03）. Conclusion：-1195G〈A poly-morphism of COX-2 gene was associated with AR or ASR in patients with IS, which may be a genetic predisposi-tion to phenotypes of AR or ASR.

关 键 词：环氧合酶-2 缺血性脑卒中 阿司匹林抵抗 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]