重金属镉胁迫对果蝇dDnmt2、dMBD2/3表达量的影响  

The effects of cadmium on expression of the dDnmt2 and dMBD2/3 genes in Drosophila melanogaster

作　　者：管德龙[1] 梁露[1] 张敏[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《应用昆虫学报》2014年第2期460-468,共9页Chinese Journal of Applied Entomology

基　　金：陕西省自然科学基础研究计划项目(2012JQ3012);中央高校基本科研业务费专项资金资助(GK201402026)

摘　　要：【目的】探明重金属镉(Cadmium,Cd)对黑腹果蝇Drosophila melanogaster DNA甲基化修饰相关基因表达的影响,初步分析镉胁迫可能导致果蝇的表观遗传变异及其可遗传性。【方法】收集8 h内羽化未交配的雌、雄果蝇,在添加不同质量浓度(0、0.9375、1.875、3.75、7.5、15.0、30.0、60.0 mg/kg)Cd的培养基中培养,以Real-time PCR定量检测亲代(F0)果蝇生殖系统、去生殖系统体细胞、整体表达量变化趋势及解除胁迫的子代(F1)果蝇DNA甲基化修饰系统相关基因(dDnmt2、dMBD2/3)在mRNA水平的表达变化。【结果】重金属镉胁迫诱导了果蝇卵巢、精巢、去卵巢雌果蝇、去精巢雄果蝇、完整雌果蝇、完整雄果蝇的dDnmt2、dMBD2/3在mRNA水平的表达上调,呈现一定剂量依赖性及雌、雄组织差异性,且这种表达变化持续至子一代。【结论】研究结果揭示了重金属镉胁迫可诱导果蝇dDnmt2、dMBD2/3表达量上调,其可能与果蝇的DNA甲基化修饰过程相关联,导致表观遗传变异并可能向子代传递。[Objectives] To investigate the effects of heavy metal cadmium （Cd） on the expression of DNA methylation-related genes, and discuss potential epigenetic variation caused by cadmium stress and its heritability in Drosophila melanogaster. [Methods] Unmated male and female flies 〈 8 h old were collected and incubated in culture medium containing different concentrations of Cd （0.0, 0.9375, 1.875, 3.75, 7.5, 15.0, 30.0, 60.0 mg/kg） for 10 days. Quantitative Real-time PCR was used to detect the mRNA expression of the DNA methylation-related genes, dDnmt2 and dMBD2/3 in the reproductive system of parental （F0） flies with Cd treatment and their offspring （F0 without Cd. [Resultsl Cadmium stress can up-regulate the expresion of dDnmt2 and dMBD2/3 in the ovaries and testes and these changes continue to the next generation （F1）. [Conclusion] Expression of dDnmt2 and dMBD2/3 in D. melanogaster is positively correlated with cadmium exposure. This may due to the DNA methylation modification process associated with epigenetic variation in D. melanogaster.

关 键 词：镉 黑腹果蝇 DNA甲基化修饰系统基因 可遗传性 

分 类 号：X174[环境科学与工程—环境科学]