硅表面分子刷图案化及生长DNA纳米管  

In-situ Growth of DNA Nanotubes from Functionalized Patterns of Poly (PEGMA) Brushes on Silicon Surface

作　　者：鄢琴[1] 肖守军[1] 

机构地区：[1]南京大学化学化工学院 配位化学国家重点实验室,南京210093

出　　处：《无机化学学报》2014年第4期741-748,共8页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.91027019)资助项目

摘　　要：结合'自上而下'和'自下而上'技术构建微纳米器件是目前纳米科学和技术领域追逐的目标之一。本文首先采用硅氢化反应在硅表面共价偶联引发聚合的活性基团,接着实施表面原子转移自由基聚合(ATRP)反应形成高分子刷poly(PEGMA),采用'自上而下'的光刻技术在硅表面制备功能化的图案,最后利用'自下而上'的DNA自组装技术在图案部分原位生长DNA纳米管。上述组装过程通过多次透射反射红外光谱、凝胶电泳、透射电镜和扫描电镜进行了检测,证实了硅芯片表面定位生长DNA纳米管的可行性。Interfacing ＂top-down＂ with ＂bottom-up＂ methods to fabricate micro- and nano-devices are one target in nano-science and nano-technology presently. Herein we demonstrate the combination of photolithography and self-assembly to grow DNA nanotubes on functionalized patterns of poly（poly（ethylene glycol）monomethacrylate） brushes grafted from a silicon chip surface （Si-g-Poly（PEGMA））. On the silicon surface, first, the bromoisobutyryl group as the polymerization initiator was introduced through hydrosilylation, then poly（PEGMA） brushes were grown in-situ by Surface Initiated Atom Transfer Radical Polymerization （SI-ATRP）, NHS（succinimidyl）-ester was generated on photolithographied poly（PEGMA） patterns and finally six-helix DNA tubes were immobilized and grown in-situ on these patterns. Multiple transmission-reflection infrared spectroscopy （MTR-IR）, gel electrophoresis, transmission electron microscopy （TEM）, and scanning electron microscopy （SEM） were used to monitor the whole process, which confirmed the feasibility of DNA serf-assembly on the functionalized patterns of a silicon chip.

关 键 词：自组装 光刻 聚合物刷 DNA纳米管 

分 类 号：O612.4[理学—无机化学] O632.5[理学—化学]