隆尧鸡腿葱中3种水溶性维生素含量分析  

Analysis of three kinds of water-soluble vitamin in Longyao jitui onions

作　　者：赵杰[1] 王菁菁[1] 丁利杰[1] 王庭欣[1] 刘峥颢[1] 

机构地区：[1]河北大学质量技术监督学院,河北保定071000

出　　处：《河北大学学报（自然科学版）》2014年第2期181-186,共6页Journal of Hebei University(Natural Science Edition)

基　　金：河北省科技支撑计划项目(12221003D)

摘　　要：采用高效液相色谱检测鸡腿葱中3种水溶性维生素（VC,VB1,VB2）的含量.以甲醇-0.05 mol/L磷酸二氢钾（pH=6.0）为流动相,进样体积20μL,柱温35℃,流速1.0 mL/min,检测波长270 nm.该方法对3种水溶性维生素的检出限均为0.05 mg/L.在其线性范围内,r达到0.999 0以上.变异系数为0.96％～1.65％,回收率测定结果为85.48％～102.40％.本方法具有准确、成本低等优点,可以用于隆尧鸡腿葱和莱芜鸡腿葱中3种水溶性维生素含量测定.测定结果显示,隆尧鸡腿葱中VC和VB2含量明显高于莱芜鸡腿葱,且有统计学差异,这将为今后隆尧鸡腿葱的开发利用提供技术支持.Based on the method of high performance liquid chromatography, this experiment detected water-soluble vitamins （VC, VB1, VB2） of Longyao jitui onions. The method was adopted with methanol and potassium dihydrogen phosphate （pH=6.0） as the moving phase. The injection volume was 20 μL, the column temperature was 35 ℃ and the flow rate was 1.0 mL/min. The detection wavelength was 270 nm. The lowest detection limit of three kinds of vitamins was 0.05 mg/L. The linear cor-relation coef- ficient was 0. 999 0 above. Precision test RSD was 0. 96%--1.65%. The recovery rate was in 85.48%-- 102.04% range. This method was accurate and suitable for the determination of three kinds of water-solu- ble vitamins in jitui onions. According to the results of the experiment, the VC and VB2 contents of the Longyao jitui onions were significantly higher than that in Laiwu jitui onions, and there was a statistical difference. This will provide technical support for the development and utilization of Longyao jitui onions.

关 键 词：隆尧鸡腿葱 水溶性维生素 高效液相色谱 

分 类 号：O657.7[理学—分析化学]