RP-HPLC测定人工虫草和天然虫草中麦角甾醇含量  

HPLC Determination of Ergosterol in Cultured Cordyceps militaris and Natural Cordyceps sinensis

作　　者：张薇薇 龚韬 韩东河 胡永君 

机构地区：[1]北京市卫生局临床药学研究所,北京100035 [2]烟台惠众生物科技有限公司,山东烟台264006

出　　处：《中国中医药信息杂志》2014年第4期67-69,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市中医药科技发展基金(JJ2009-14)

摘　　要：目的：建立反相高效液相色谱法测定人工虫草和天然虫草中麦角甾醇的含量。方法采用Diamonsil C18（2）色谱柱（150 mm＆#215;4.6 mm,5μm）,流动相为甲醇-水（98∶2）,检测波长280 nm,柱温25℃,流速1.0 mL/min。结果麦角甾醇进样量在0.1977～3.9549μg 范围内与峰面积呈良好线性关系（r＝1.000）,平均加样回收率为99.51%,RSD＝0.56%（n＝9）。结论本方法操作简便、灵敏、准确,具有良好的重复性和稳定性,适用于人工虫草和天然虫草中麦角甾醇的含量测定。Objective To develop an HPLC method for determination of content of ergosterol in cultured Cordyceps militaris and natural Cordyceps sinensis. Methods Diamonsil C18（2） column （150 mm＆#215;4.6 mm, 5 μm） was used, with methanol-water （98∶2） as mobile phase, column temperature of 25 ℃ , flow rate of 1.0 mL/min, and detection wavelength of 280 nm. Results The linear range of ergosterol was 0.197 7-3.954 9 μg （r=1.000）, and avarage recovery rate was 99.51%, RSD was 0.56% （n=9）. Conclusion This method is effective, sensitive and accurate with high repeatability and stability, which is helpful for the determination of ergosterol in the cultured Cordyceps and natural Cordyceps.

关 键 词：麦角甾醇 人工北虫草 发酵虫草菌粉 冬虫夏草 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]